Field emission scanning electron microscopy of biofilm-growing bacteria involved in nosocomial infections

Claudia Vuotto, Gianfranco Donelli

Research output: Contribution to journalArticlepeer-review

Abstract

Scanning electron microscopy (SEM) provides useful information on the shape, size, and localization within the biofilm of single bacteria as well as on the steps of biofilm formation process, on bacterial interactions, and on production of extracellular polymeric substances. When biofilms are constituted by microbial species involved in health care-associated infections, information provided by SEM can be fruitfully used not only for basic researches but also for diagnostic purposes. The protocols currently used in our laboratory for biofilm investigation by SEM are reported here. Particularly, the procedures to fix, dehydrate, and metalize in vitro-developed biofilms or ex vivo clinical specimens colonized by biofilm-growing microorganisms are described as well as the advantages of the observation of these samples by field emission scanning electron microscopy.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume1147
DOIs
Publication statusPublished - 2014

Keywords

  • Biofilm ultrastructure
  • FESEM
  • Medical devices
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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