Field evaluation of capillary blood samples as a collection specimen for the rapid diagnosis of ebola virus infection during an outbreak emergency

Thomas Strecker, Bernadett Palyi, Heinz Ellerbrok, Sylvie Jonckheere, Hilde De Clerck, Joseph Akoi Bore, Martin Gabriel, Kilian Stoecker, Markus Eickmann, Michel Van Herp, Pierre Formenty, Antonino Di Caro, Stephan Becker

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background. Reliable reverse transcription polymerase chain reaction (RT-PCR)-based diagnosis of Ebola virus infection currently requires a blood sample obtained by intravenous puncture. During the current Ebola outbreak in Guinea, we evaluated the usability of capillary blood samples collected from fingersticks of patients suspected of having Ebola virus disease (EVD) for field diagnostics during an outbreak emergency. Methods. A total of 120 venous and capillary blood samples were collected from 53 patients admitted to the Ebola Treatment Centre in Guéckédou, Guinea, between July and August 2014. All sample specimens were analyzed by RT-PCR using the RealStar Filovirus Screen RT-PCR Kit 1.0 from altona Diagnostics (Germany). We compared samples obtained by venipuncture and those obtained by capillary blood sampling absorbed onto swab devices. Results. The resulting sensitivity and specificity of tests performed with capillary blood samples were 86.8% (95% confidence interval [CI], 71.9%-95.6%; 33/38 patients) and 100% (95% CI, 84.6%-100%; 22/22 patients), respectively. Conclusions. Our data suggest that capillary blood samples could serve as an alternative to venous blood samples for the diagnosis of EVD in resource-limited settings during a crisis. This can be of particular advantage in cases when venipuncture is difficult to perform-for example, with newborns and infants or when adult patients reject venipuncture for cultural or religious reasons.

Original languageEnglish
Pages (from-to)669-675
Number of pages7
JournalClinical Infectious Diseases
Volume61
Issue number5
DOIs
Publication statusPublished - 2015

Fingerprint

Ebola Hemorrhagic Fever
Specimen Handling
Disease Outbreaks
Emergencies
Phlebotomy
Guinea
Reverse Transcription
Polymerase Chain Reaction
Confidence Intervals
Punctures
Germany
Newborn Infant
Sensitivity and Specificity
Equipment and Supplies

Keywords

  • Capillary blood
  • Ebola virus
  • Ebola virus disease
  • Field diagnostic

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Field evaluation of capillary blood samples as a collection specimen for the rapid diagnosis of ebola virus infection during an outbreak emergency. / Strecker, Thomas; Palyi, Bernadett; Ellerbrok, Heinz; Jonckheere, Sylvie; De Clerck, Hilde; Bore, Joseph Akoi; Gabriel, Martin; Stoecker, Kilian; Eickmann, Markus; Van Herp, Michel; Formenty, Pierre; Di Caro, Antonino; Becker, Stephan.

In: Clinical Infectious Diseases, Vol. 61, No. 5, 2015, p. 669-675.

Research output: Contribution to journalArticle

Strecker, T, Palyi, B, Ellerbrok, H, Jonckheere, S, De Clerck, H, Bore, JA, Gabriel, M, Stoecker, K, Eickmann, M, Van Herp, M, Formenty, P, Di Caro, A & Becker, S 2015, 'Field evaluation of capillary blood samples as a collection specimen for the rapid diagnosis of ebola virus infection during an outbreak emergency', Clinical Infectious Diseases, vol. 61, no. 5, pp. 669-675. https://doi.org/10.1093/cid/civ397
Strecker, Thomas ; Palyi, Bernadett ; Ellerbrok, Heinz ; Jonckheere, Sylvie ; De Clerck, Hilde ; Bore, Joseph Akoi ; Gabriel, Martin ; Stoecker, Kilian ; Eickmann, Markus ; Van Herp, Michel ; Formenty, Pierre ; Di Caro, Antonino ; Becker, Stephan. / Field evaluation of capillary blood samples as a collection specimen for the rapid diagnosis of ebola virus infection during an outbreak emergency. In: Clinical Infectious Diseases. 2015 ; Vol. 61, No. 5. pp. 669-675.
@article{269cab2c29844efe846044953204d358,
title = "Field evaluation of capillary blood samples as a collection specimen for the rapid diagnosis of ebola virus infection during an outbreak emergency",
abstract = "Background. Reliable reverse transcription polymerase chain reaction (RT-PCR)-based diagnosis of Ebola virus infection currently requires a blood sample obtained by intravenous puncture. During the current Ebola outbreak in Guinea, we evaluated the usability of capillary blood samples collected from fingersticks of patients suspected of having Ebola virus disease (EVD) for field diagnostics during an outbreak emergency. Methods. A total of 120 venous and capillary blood samples were collected from 53 patients admitted to the Ebola Treatment Centre in Gu{\'e}ck{\'e}dou, Guinea, between July and August 2014. All sample specimens were analyzed by RT-PCR using the RealStar Filovirus Screen RT-PCR Kit 1.0 from altona Diagnostics (Germany). We compared samples obtained by venipuncture and those obtained by capillary blood sampling absorbed onto swab devices. Results. The resulting sensitivity and specificity of tests performed with capillary blood samples were 86.8{\%} (95{\%} confidence interval [CI], 71.9{\%}-95.6{\%}; 33/38 patients) and 100{\%} (95{\%} CI, 84.6{\%}-100{\%}; 22/22 patients), respectively. Conclusions. Our data suggest that capillary blood samples could serve as an alternative to venous blood samples for the diagnosis of EVD in resource-limited settings during a crisis. This can be of particular advantage in cases when venipuncture is difficult to perform-for example, with newborns and infants or when adult patients reject venipuncture for cultural or religious reasons.",
keywords = "Capillary blood, Ebola virus, Ebola virus disease, Field diagnostic",
author = "Thomas Strecker and Bernadett Palyi and Heinz Ellerbrok and Sylvie Jonckheere and {De Clerck}, Hilde and Bore, {Joseph Akoi} and Martin Gabriel and Kilian Stoecker and Markus Eickmann and {Van Herp}, Michel and Pierre Formenty and {Di Caro}, Antonino and Stephan Becker",
year = "2015",
doi = "10.1093/cid/civ397",
language = "English",
volume = "61",
pages = "669--675",
journal = "Clinical Infectious Diseases",
issn = "1058-4838",
publisher = "NLM (Medline)",
number = "5",

}

TY - JOUR

T1 - Field evaluation of capillary blood samples as a collection specimen for the rapid diagnosis of ebola virus infection during an outbreak emergency

AU - Strecker, Thomas

AU - Palyi, Bernadett

AU - Ellerbrok, Heinz

AU - Jonckheere, Sylvie

AU - De Clerck, Hilde

AU - Bore, Joseph Akoi

AU - Gabriel, Martin

AU - Stoecker, Kilian

AU - Eickmann, Markus

AU - Van Herp, Michel

AU - Formenty, Pierre

AU - Di Caro, Antonino

AU - Becker, Stephan

PY - 2015

Y1 - 2015

N2 - Background. Reliable reverse transcription polymerase chain reaction (RT-PCR)-based diagnosis of Ebola virus infection currently requires a blood sample obtained by intravenous puncture. During the current Ebola outbreak in Guinea, we evaluated the usability of capillary blood samples collected from fingersticks of patients suspected of having Ebola virus disease (EVD) for field diagnostics during an outbreak emergency. Methods. A total of 120 venous and capillary blood samples were collected from 53 patients admitted to the Ebola Treatment Centre in Guéckédou, Guinea, between July and August 2014. All sample specimens were analyzed by RT-PCR using the RealStar Filovirus Screen RT-PCR Kit 1.0 from altona Diagnostics (Germany). We compared samples obtained by venipuncture and those obtained by capillary blood sampling absorbed onto swab devices. Results. The resulting sensitivity and specificity of tests performed with capillary blood samples were 86.8% (95% confidence interval [CI], 71.9%-95.6%; 33/38 patients) and 100% (95% CI, 84.6%-100%; 22/22 patients), respectively. Conclusions. Our data suggest that capillary blood samples could serve as an alternative to venous blood samples for the diagnosis of EVD in resource-limited settings during a crisis. This can be of particular advantage in cases when venipuncture is difficult to perform-for example, with newborns and infants or when adult patients reject venipuncture for cultural or religious reasons.

AB - Background. Reliable reverse transcription polymerase chain reaction (RT-PCR)-based diagnosis of Ebola virus infection currently requires a blood sample obtained by intravenous puncture. During the current Ebola outbreak in Guinea, we evaluated the usability of capillary blood samples collected from fingersticks of patients suspected of having Ebola virus disease (EVD) for field diagnostics during an outbreak emergency. Methods. A total of 120 venous and capillary blood samples were collected from 53 patients admitted to the Ebola Treatment Centre in Guéckédou, Guinea, between July and August 2014. All sample specimens were analyzed by RT-PCR using the RealStar Filovirus Screen RT-PCR Kit 1.0 from altona Diagnostics (Germany). We compared samples obtained by venipuncture and those obtained by capillary blood sampling absorbed onto swab devices. Results. The resulting sensitivity and specificity of tests performed with capillary blood samples were 86.8% (95% confidence interval [CI], 71.9%-95.6%; 33/38 patients) and 100% (95% CI, 84.6%-100%; 22/22 patients), respectively. Conclusions. Our data suggest that capillary blood samples could serve as an alternative to venous blood samples for the diagnosis of EVD in resource-limited settings during a crisis. This can be of particular advantage in cases when venipuncture is difficult to perform-for example, with newborns and infants or when adult patients reject venipuncture for cultural or religious reasons.

KW - Capillary blood

KW - Ebola virus

KW - Ebola virus disease

KW - Field diagnostic

UR - http://www.scopus.com/inward/record.url?scp=84940953877&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84940953877&partnerID=8YFLogxK

U2 - 10.1093/cid/civ397

DO - 10.1093/cid/civ397

M3 - Article

AN - SCOPUS:84940953877

VL - 61

SP - 669

EP - 675

JO - Clinical Infectious Diseases

JF - Clinical Infectious Diseases

SN - 1058-4838

IS - 5

ER -