Abstract
FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.
| Original language | English |
|---|---|
| Pages (from-to) | 183-186 |
| Number of pages | 4 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Volume | 79 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2014 |
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Keywords
- FilmArray
- Multiple PCR tests
- Respiratory viruses
ASJC Scopus subject areas
- Microbiology (medical)
- Infectious Diseases
- Medicine(all)
Cite this
FilmArray® respiratory panel performance in respiratory samples from neonatal care units. / Piralla, Antonio; Lunghi, Giovanna; Percivalle, Elena; Viganò, Cristina; Nasta, Teresa; Pugni, Lorenza; Mosca, Fabio; Stronati, Mauro; Torresani, Erminio; Baldanti, Fausto.
In: Diagnostic Microbiology and Infectious Disease, Vol. 79, No. 2, 2014, p. 183-186.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - FilmArray® respiratory panel performance in respiratory samples from neonatal care units
AU - Piralla, Antonio
AU - Lunghi, Giovanna
AU - Percivalle, Elena
AU - Viganò, Cristina
AU - Nasta, Teresa
AU - Pugni, Lorenza
AU - Mosca, Fabio
AU - Stronati, Mauro
AU - Torresani, Erminio
AU - Baldanti, Fausto
PY - 2014
Y1 - 2014
N2 - FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.
AB - FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.
KW - FilmArray
KW - Multiple PCR tests
KW - Respiratory viruses
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U2 - 10.1016/j.diagmicrobio.2014.02.010
DO - 10.1016/j.diagmicrobio.2014.02.010
M3 - Article
VL - 79
SP - 183
EP - 186
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 2
ER -