FilmArray® respiratory panel performance in respiratory samples from neonatal care units

Antonio Piralla, Giovanna Lunghi, Elena Percivalle, Cristina Viganò, Teresa Nasta, Lorenza Pugni, Fabio Mosca, Mauro Stronati, Erminio Torresani, Fausto Baldanti

Research output: Contribution to journalArticlepeer-review

Abstract

FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.

Original languageEnglish
Pages (from-to)183-186
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume79
Issue number2
DOIs
Publication statusPublished - 2014

Keywords

  • FilmArray
  • Multiple PCR tests
  • Respiratory viruses

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Medicine(all)

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