Fine characterization of mitral valve glycosaminoglycans and their modification with degenerative disease

Luca Dainese, GianLuca Polvani, Fabio Barili, Francesca Maccari, Anna Guarino, Francesco Alamanni, Marco Zanobini, Paolo Biglioli, Nicola Volpi

Research output: Contribution to journalArticle

Abstract

Background: The levels and fine structure of complex polysaccharides, glycosaminoglycans (GAGs), were determined in segments of the posterior mitral valve leaflet (MVL) taken from 15 patients affected by mitral regurgitation and degenerative disease and were compared with segments from 15 multiorgan donors. Methods: MVL GAGs were analyzed by agarose gel electrophoresis, and by HPLC and fluorophore-assisted carbohydrate electrophoresis to evaluate disaccharide patterns after treatment with chondroitinase ABC. Results: GAGs from the control group were composed of approximately 37% hyaluronic acid and 63% chondroitin sulfate/dermatan sulfate with a charge density of approximately 0.61. Chondroitin sulfate/dermatan sulfate polymers contained approximately 23% of the disaccharide sulfated in position 6 on N-acetyl-galactosamine, ∼38% of the 4-sulfated disaccharide and ∼2% of the non-sulfated disaccharide (with a 4-sulfated/6-sulfated ratio of 1.7). The total amount of GAGs was 0.66 μg/mg tissue. The total amount of GAGs in patients suffering from mitral regurgitation and degenerative disease was approximately 51.5% higher (although the difference was not significant, probably because of the low number of subjects enrolled in the study). However, significantly higher hyaluronic acid content (approx. +38%, p

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalClinical Chemistry and Laboratory Medicine
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 1 2007

Keywords

  • Chondroitin sulfate
  • Degenerative disease
  • Glycosaminoglycans
  • Hyaluronic acid
  • Mitral valve

ASJC Scopus subject areas

  • Clinical Biochemistry

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