TY - JOUR
T1 - First detection of SARS-CoV-2 in untreated wastewaters in Italy
AU - La Rosa, Giuseppina
AU - Iaconelli, Marcello
AU - Mancini, Pamela
AU - Bonanno Ferraro, Giusy
AU - Veneri, Carolina
AU - Bonadonna, Lucia
AU - Lucentini, Luca
AU - Suffredini, Elisabetta
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2020/9/20
Y1 - 2020/9/20
N2 - Several studies have demonstrated the advantages of environmental surveillance through the monitoring of sewage for the assessment of viruses circulating in a given community (wastewater-based epidemiology, WBE). During the COVID-19 public health emergency, many reports have described the presence of SARS-CoV-2 RNA in stools from COVID-19 patients, and a few studies reported the occurrence of SARS-CoV-2 in wastewaters worldwide. Italy is among the world's worst-affected countries in the COVID-19 pandemic, but so far there are no studies assessing the presence of SARS-CoV-2 in Italian wastewaters. To this aim, twelve influent sewage samples, collected between February and April 2020 from Wastewater Treatment Plants in Milan and Rome, were tested adapting, for concentration, the standard WHO procedure for Poliovirus surveillance. Molecular analysis was undertaken with three nested protocols, including a newly designed SARS-CoV-2 specific primer set. SARS-CoV-2 RNA detection was accomplished in volumes of 250 ml of wastewaters collected in areas of high (Milan) and low (Rome) epidemic circulation, according to clinical data. Overall, 6 out of 12 samples were positive. One of the positive results was obtained in a Milan wastewater sample collected a few days after the first notified Italian case of autochthonous SARS-CoV-2. The study confirms that WBE has the potential to be applied to SARS-CoV-2 as a sensitive tool to study spatial and temporal trends of virus circulation in the population.
AB - Several studies have demonstrated the advantages of environmental surveillance through the monitoring of sewage for the assessment of viruses circulating in a given community (wastewater-based epidemiology, WBE). During the COVID-19 public health emergency, many reports have described the presence of SARS-CoV-2 RNA in stools from COVID-19 patients, and a few studies reported the occurrence of SARS-CoV-2 in wastewaters worldwide. Italy is among the world's worst-affected countries in the COVID-19 pandemic, but so far there are no studies assessing the presence of SARS-CoV-2 in Italian wastewaters. To this aim, twelve influent sewage samples, collected between February and April 2020 from Wastewater Treatment Plants in Milan and Rome, were tested adapting, for concentration, the standard WHO procedure for Poliovirus surveillance. Molecular analysis was undertaken with three nested protocols, including a newly designed SARS-CoV-2 specific primer set. SARS-CoV-2 RNA detection was accomplished in volumes of 250 ml of wastewaters collected in areas of high (Milan) and low (Rome) epidemic circulation, according to clinical data. Overall, 6 out of 12 samples were positive. One of the positive results was obtained in a Milan wastewater sample collected a few days after the first notified Italian case of autochthonous SARS-CoV-2. The study confirms that WBE has the potential to be applied to SARS-CoV-2 as a sensitive tool to study spatial and temporal trends of virus circulation in the population.
KW - Coronavirus
KW - COVID-19
KW - SARS-CoV-2
KW - Sewage
KW - Surveillance
KW - Wastewater
UR - http://www.scopus.com/inward/record.url?scp=85084959240&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85084959240&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2020.139652
DO - 10.1016/j.scitotenv.2020.139652
M3 - Article
C2 - 32464333
AN - SCOPUS:85084959240
VL - 736
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 139652
ER -