First molecular method for discriminating between Candida africana, Candida albicans, and Candida dubliniensis by using hwp1 gene

Orazio Romeo, Giuseppe Criseo

Research output: Contribution to journalArticlepeer-review

Abstract

The authors report here a polymerase chain reaction-based assay using a single primer pair (CR-f/CR-r), allowing discrimination between Candida africana and Candida albicans by using the hwp1 gene. The method also identifies Candida dubliniensis because it produces 3 different DNA fragments: approximately 700 bp for C. africana, 941 bp for C. albicans, and 569 for C. dubliniensis.

Original languageEnglish
Pages (from-to)230-233
Number of pages4
JournalDiagnostic Microbiology and Infectious Disease
Volume62
Issue number2
DOIs
Publication statusPublished - Oct 2008

Keywords

  • Candida africana
  • Candida albicans
  • Candida dubliniensis
  • hwp1 gene
  • PCR-based assay

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology
  • Parasitology
  • Virology
  • Immunology and Allergy
  • Infectious Diseases

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