First steps to define murine amniotic fluid stem cell microenvironment

E. Bertin, M. Piccoli, C. Franzin, G. Spiro, S. Donà, A. Dedja, F. Schiavi, Elisa Taschin, P. Bonaldo, P. Braghetta, P. De Coppi, M. Pozzobon

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Stem cell niche refers to the microenvironment where stem cells reside in living organisms. Several elements define the niche and regulate stem cell characteristics, such as stromal support cells, gap junctions, soluble factors, extracellular matrix proteins, blood vessels and neural inputs. In the last years, different studies demonstrated the presence of cKit+ cells in human and murine amniotic fluid, which have been defined as amniotic fluid stem (AFS) cells. Firstly, we characterized the murine cKit+ cells present both in the amniotic fluid and in the amnion. Secondly, to analyze the AFS cell microenvironment, we injected murine YFP+ embryonic stem cells (ESC) into the amniotic fluid of E13.5 wild type embryos. Four days after transplantation we found that YFP + sorted cells maintained the expression of pluripotency markers and that ESC adherent to the amnion were more similar to original ESC in respect to those isolated from the amniotic fluid. Moreover, cytokines evaluation and oxygen concentration analysis revealed in this microenvironment the presence of factors that are considered key regulators in stem cell niches. This is the first indication that AFS cells reside in a microenvironment that possess specific characteristics able to maintain stemness of resident and exogenous stem cells.

Original languageEnglish
Article number37080
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Nov 15 2016

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Stem Cell Niche
Amniotic Fluid
Embryonic Stem Cells
Amnion
Stem Cells
Intercellular Junctions
Extracellular Matrix Proteins
Gap Junctions
Stromal Cells
Blood Vessels
Embryonic Structures
Transplantation
Cytokines
Oxygen

ASJC Scopus subject areas

  • General

Cite this

Bertin, E., Piccoli, M., Franzin, C., Spiro, G., Donà, S., Dedja, A., ... Pozzobon, M. (2016). First steps to define murine amniotic fluid stem cell microenvironment. Scientific Reports, 6, [37080]. https://doi.org/10.1038/srep37080

First steps to define murine amniotic fluid stem cell microenvironment. / Bertin, E.; Piccoli, M.; Franzin, C.; Spiro, G.; Donà, S.; Dedja, A.; Schiavi, F.; Taschin, Elisa; Bonaldo, P.; Braghetta, P.; De Coppi, P.; Pozzobon, M.

In: Scientific Reports, Vol. 6, 37080, 15.11.2016.

Research output: Contribution to journalArticle

Bertin, E, Piccoli, M, Franzin, C, Spiro, G, Donà, S, Dedja, A, Schiavi, F, Taschin, E, Bonaldo, P, Braghetta, P, De Coppi, P & Pozzobon, M 2016, 'First steps to define murine amniotic fluid stem cell microenvironment', Scientific Reports, vol. 6, 37080. https://doi.org/10.1038/srep37080
Bertin E, Piccoli M, Franzin C, Spiro G, Donà S, Dedja A et al. First steps to define murine amniotic fluid stem cell microenvironment. Scientific Reports. 2016 Nov 15;6. 37080. https://doi.org/10.1038/srep37080
Bertin, E. ; Piccoli, M. ; Franzin, C. ; Spiro, G. ; Donà, S. ; Dedja, A. ; Schiavi, F. ; Taschin, Elisa ; Bonaldo, P. ; Braghetta, P. ; De Coppi, P. ; Pozzobon, M. / First steps to define murine amniotic fluid stem cell microenvironment. In: Scientific Reports. 2016 ; Vol. 6.
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