FISH analysis in cell touch preparations and cytological specimens from formalin-fixed fetal autopsies

Francesco Rivasi, Laura Schirosi, Stefania Bettelli, Nazzarena Bigiani, Carlo Curatola

Research output: Contribution to journalArticlepeer-review

Abstract

Postmortem studies on still-borns and miscarriages are impor tant to define the sex and eventually the morphologic anoma lies correlated to chromosomal aberrations. When the condi tions for carrying out a cytogenetic study do not exist, these chromosomal alterations can be investigated by nucleic acid fluorescent in situ hybridization (FISH), which can be per formed on interphase nuclei, usually on formalin-fixed paraffin embedded tissues or on fresh cytological specimens. The objec tive of the present study is to prove whether this technique can be successfully applied to formalin-fixed cell touch prepara tions and cytological specimens obtained from foetal autopsies. The study was carried out 12 abortions some of which were spontaneous and some of which were therapeutic. The materi als were formalin-fixed. Cell touch preparations and cytologi cal specimens were obtained. The FISH was performed using XIY probes (Vysis) and the Aneuvysion Kit (05J38-030, Vysis), the probes being for chromosomes 13/21 and X/Y/18. To verify the reliability of the technique, the same reactions were also performed on fresh analogous materials. The slides were evaluable, and the probes hybridized to interphase nuclei showed distinct signals. All the samples were adequate for FISH analysis without any notable difference in the results. Moreover, it is technically possible to perform this analysis not only on fresh but particularly on formalin-fixed cytological specimens. On the other hand, the use of this type of cytologi cal samples, as compared to formalin-fixed and paraffin em bedded tissue sections, has the advantage of presenting intact, noncut nuclei with preserved cytomorphology, avoiding the problems of overlapping nuclei and making the identification of the real chromosomal arrangement easier.

Original languageEnglish
Pages (from-to)633-636
Number of pages4
JournalDiagnostic Cytopathology
Volume36
Issue number9
DOIs
Publication statusPublished - Sep 2008

Keywords

  • Cell touch preparations
  • Cytological specimens
  • FISH
  • Formalin-fixed fetal autopsies

ASJC Scopus subject areas

  • Anatomy

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