FISH analysis of hematological neoplasias with 1p36 rearrangements allows the definition of a cluster of 2.5 Mb included in the minimal region deleted in 1p36 deletion syndrome

Idoya Lahortiga, Iria Vázquez, Elena Belloni, José P. Román, Patrizia Gasparini, Francisco J. Novo, Isabel Zudaire, Pier G. Pelicci, Jesús M. Hernández, María J. Calasanz, María D. Odero

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Abstract

Rearrangements in the distal region of the short arm of chromosome 1 are recurrent aberrations in a broad spectrum of human neoplasias. However, neither the location of the breakpoints (BP) on 1p36 nor the candidate genes have been fully determined. We have characterized, by fluorescence in situ hybridization (FISH), the BP in 26 patients with hematological neoplasias and 1p36 rearrangements in the G-banding karyotype. FISH allowed a better characterization of all samples analyzed. Nine cases (35%) showed reciprocal translocations, 15 (58%) unbalanced rearrangements, and two (7%) deletions. We describe two new recurrent aberrations. In 18 of the 26 cases analyzed the BP were located in band 1p36, which is 25.5 Mb long. In 14 of these 18 cases (78%) and without distinction between myeloid and lymphoid neoplasias, the BP clustered in a 2.5 Mb region located between 1p36.32 and the telomere. Interestingly, this region is contained in the 10.5 Mb cluster on 1p36.22-1pter defined in cases with 1p36 deletion syndrome. The 2.5 Mb region, located on 1p36.32-1pter, has a higher frequency of occurrence of tandem repeats and segmental duplications larger than 1 kb, when compared with the 25.5 Mb of the complete 1p36 band. This could explain its proneness for involvement in chromosomal rearrangements in hematological neoplasias.

Original languageEnglish
Pages (from-to)476-485
Number of pages10
JournalHuman Genetics
Volume116
Issue number6
DOIs
Publication statusPublished - May 2005

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Fluorescence In Situ Hybridization
Neoplasms
Genomic Segmental Duplications
Tandem Repeat Sequences
Chromosomes, Human, Pair 1
Telomere
Karyotype
Chromosome 1p36 Deletion Syndrome
Genes

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

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FISH analysis of hematological neoplasias with 1p36 rearrangements allows the definition of a cluster of 2.5 Mb included in the minimal region deleted in 1p36 deletion syndrome. / Lahortiga, Idoya; Vázquez, Iria; Belloni, Elena; Román, José P.; Gasparini, Patrizia; Novo, Francisco J.; Zudaire, Isabel; Pelicci, Pier G.; Hernández, Jesús M.; Calasanz, María J.; Odero, María D.

In: Human Genetics, Vol. 116, No. 6, 05.2005, p. 476-485.

Research output: Contribution to journalArticle

Lahortiga, Idoya ; Vázquez, Iria ; Belloni, Elena ; Román, José P. ; Gasparini, Patrizia ; Novo, Francisco J. ; Zudaire, Isabel ; Pelicci, Pier G. ; Hernández, Jesús M. ; Calasanz, María J. ; Odero, María D. / FISH analysis of hematological neoplasias with 1p36 rearrangements allows the definition of a cluster of 2.5 Mb included in the minimal region deleted in 1p36 deletion syndrome. In: Human Genetics. 2005 ; Vol. 116, No. 6. pp. 476-485.
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abstract = "Rearrangements in the distal region of the short arm of chromosome 1 are recurrent aberrations in a broad spectrum of human neoplasias. However, neither the location of the breakpoints (BP) on 1p36 nor the candidate genes have been fully determined. We have characterized, by fluorescence in situ hybridization (FISH), the BP in 26 patients with hematological neoplasias and 1p36 rearrangements in the G-banding karyotype. FISH allowed a better characterization of all samples analyzed. Nine cases (35{\%}) showed reciprocal translocations, 15 (58{\%}) unbalanced rearrangements, and two (7{\%}) deletions. We describe two new recurrent aberrations. In 18 of the 26 cases analyzed the BP were located in band 1p36, which is 25.5 Mb long. In 14 of these 18 cases (78{\%}) and without distinction between myeloid and lymphoid neoplasias, the BP clustered in a 2.5 Mb region located between 1p36.32 and the telomere. Interestingly, this region is contained in the 10.5 Mb cluster on 1p36.22-1pter defined in cases with 1p36 deletion syndrome. The 2.5 Mb region, located on 1p36.32-1pter, has a higher frequency of occurrence of tandem repeats and segmental duplications larger than 1 kb, when compared with the 25.5 Mb of the complete 1p36 band. This could explain its proneness for involvement in chromosomal rearrangements in hematological neoplasias.",
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AU - Belloni, Elena

AU - Román, José P.

AU - Gasparini, Patrizia

AU - Novo, Francisco J.

AU - Zudaire, Isabel

AU - Pelicci, Pier G.

AU - Hernández, Jesús M.

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AU - Odero, María D.

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