Fishmeal replacement with hermetia illucens meal in aquafeeds: Effects on zebrafish growth performances, intestinal morphometry, and enzymology

Baldassare Fronte, Rosario Licitra, Carlo Bibbiani, Lucia Casini, Mahanama De Zoysa, Vincenzo Miragliotta, Simona Sagona, Francesca Coppola, Letizia Brogi, Francesca Abramo

Research output: Contribution to journalArticlepeer-review

Abstract

Fishmeal (FM) is still the most important protein source in aquafeeds. However, due to the reduction of wild fish stocks used for FM production, its manufacturing it is now unsustainable. Insect meal represents a valid alternative to FM, due to the low carbon footprint of its production and its high nutritional value. The aim of this study was to investigate the potentials of replacing FM with black soldier fly (Hermetia illucens) meal (HIM) in aquafeeds, using zebrafish as the animal model. Four diets were formulated with increasing HIM/FM replacement rate (the control diet contained 20% FM). Mortality rates, growth performances, and feed consumptions were measured, and fish intestine samples were collected for histological and enzymatic analysis. After 49 days, all groups almost tripled their initial body weight (cumulative body weight gain ranged between 143.9 and 155.2 mg), and no statistically significant differences among treatments were observed in relation to growth performances and histological traits. Contrarily, trypsin, alkaline phosphatases, and alpha amylase–glucoamylase activities were significantly reduced when the FM replacement rate was increased. In conclusion, HIM may represent an alternative to FM, since no adverse effects were observed when it was included up to 20% in the zebrafish diet, even when replacing 100% of the FM.

Original languageEnglish
Article number28
JournalFishes
Volume6
Issue number3
DOIs
Publication statusPublished - Sep 2021

Keywords

  • Black soldier fly
  • Digestive enzymes
  • Fishmeal
  • Growth performance
  • Hermetia illucens
  • Insect meal
  • Intestinal morphometry
  • Nutrition
  • Protein source
  • Zebrafish

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Fingerprint

Dive into the research topics of 'Fishmeal replacement with hermetia illucens meal in aquafeeds: Effects on zebrafish growth performances, intestinal morphometry, and enzymology'. Together they form a unique fingerprint.

Cite this