FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy

Lucilla Bongiorno-Borbone, Antonella De Cola, Patrizia Vernole, Livio Finos, Daniela Barcaroli, Richard A. Knight, Gerry Melino, Vincenzo De Laurenzi

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Cajal Bodies are one of many specialised organelles contained in the eukaryotic cell nucleus, and are involved in a number of functions, including regulation of replication-dependent histone gene transcription. In normal diploid cells their number varies between 0 and 4 depending on the cell cycle phase, although in cancer cell lines their number is extremely variable and it has been suggested that it correlates with cell ploidy. Here we show that in mammalian cells, as in Drosophila, two distinct though functionally related bodies exist: a histone gene locus body and a Cajal Body. The first one can be detected using FLASH or NPAT as markers while the second is labelled using antibodies against Coilin. Only the number of FLASH/NPAT histone gene locus bodies correlates with ploidy and only these organelles appear to be regulated during the cell cycle. Finally, we show that the two organelles completely co-localize during the S phase of the cell cycle.

Original languageEnglish
Pages (from-to)2357-2367
Number of pages11
JournalCell Cycle
Volume7
Issue number15
Publication statusPublished - Aug 1 2008

Fingerprint

Ploidies
Organelles
Histones
Cell Cycle
Cells
Genes
Eukaryotic Cells
Cell Nucleus
Diploidy
S Phase
Drosophila
Cell Count
Cell Line
Antibodies
Transcription
Neoplasms

Keywords

  • Cajal bodies
  • Coilin
  • FLASH
  • NPAT
  • Ploidy

ASJC Scopus subject areas

  • Cell Biology
  • Biochemistry
  • Molecular Biology

Cite this

Bongiorno-Borbone, L., De Cola, A., Vernole, P., Finos, L., Barcaroli, D., Knight, R. A., ... De Laurenzi, V. (2008). FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy. Cell Cycle, 7(15), 2357-2367.

FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy. / Bongiorno-Borbone, Lucilla; De Cola, Antonella; Vernole, Patrizia; Finos, Livio; Barcaroli, Daniela; Knight, Richard A.; Melino, Gerry; De Laurenzi, Vincenzo.

In: Cell Cycle, Vol. 7, No. 15, 01.08.2008, p. 2357-2367.

Research output: Contribution to journalArticle

Bongiorno-Borbone, L, De Cola, A, Vernole, P, Finos, L, Barcaroli, D, Knight, RA, Melino, G & De Laurenzi, V 2008, 'FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy', Cell Cycle, vol. 7, no. 15, pp. 2357-2367.
Bongiorno-Borbone L, De Cola A, Vernole P, Finos L, Barcaroli D, Knight RA et al. FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy. Cell Cycle. 2008 Aug 1;7(15):2357-2367.
Bongiorno-Borbone, Lucilla ; De Cola, Antonella ; Vernole, Patrizia ; Finos, Livio ; Barcaroli, Daniela ; Knight, Richard A. ; Melino, Gerry ; De Laurenzi, Vincenzo. / FLASH and NPAT positive but not Coilin positive Cajal Bodies correlate with cell ploidy. In: Cell Cycle. 2008 ; Vol. 7, No. 15. pp. 2357-2367.
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