Abstract
Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROα, MCP-1, RANTES, MIP-1α and MIP-1β expression was evaluated by flow cytometric analysis and RT-PCR in chondrocytes isolated from cartilage specimens obtained from patients with osteoarthritis and rheumatoid arthritis and multiorgan donors as normal controls. All the chemokines except RANTES were found in normal chondrocytes, with different degrees of staining intensity. In osteoarthritis and rheumatoid arthritis patients, an enhancement of IL-8, GROα, MIP-1α and MIP-1β was observed. Copyright (C) 1999 Federation of European Biochemical Societies.
| Original language | English |
|---|---|
| Pages (from-to) | 238-242 |
| Number of pages | 5 |
| Journal | FEBS Letters |
| Volume | 455 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 23 1999 |
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Keywords
- Chemokine
- Chondrocyte
- Flow cytometry
- Osteoarthritis
- Rheumatoid arthritis
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Flow cytometric analysis of intracellular chemokines in chondrocytes in vivo : Constitutive expression and enhancement in osteoarthritis and rheumatoid arthritis. / Borzì, Rosa Maria; Mazzetti, Ilaria; Macor, Sandro; Silvestri, Tania; Bassi, Alessandra; Cattini, Luca; Facchini, Andrea.
In: FEBS Letters, Vol. 455, No. 3, 23.07.1999, p. 238-242.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow cytometric analysis of intracellular chemokines in chondrocytes in vivo
T2 - Constitutive expression and enhancement in osteoarthritis and rheumatoid arthritis
AU - Borzì, Rosa Maria
AU - Mazzetti, Ilaria
AU - Macor, Sandro
AU - Silvestri, Tania
AU - Bassi, Alessandra
AU - Cattini, Luca
AU - Facchini, Andrea
PY - 1999/7/23
Y1 - 1999/7/23
N2 - Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROα, MCP-1, RANTES, MIP-1α and MIP-1β expression was evaluated by flow cytometric analysis and RT-PCR in chondrocytes isolated from cartilage specimens obtained from patients with osteoarthritis and rheumatoid arthritis and multiorgan donors as normal controls. All the chemokines except RANTES were found in normal chondrocytes, with different degrees of staining intensity. In osteoarthritis and rheumatoid arthritis patients, an enhancement of IL-8, GROα, MIP-1α and MIP-1β was observed. Copyright (C) 1999 Federation of European Biochemical Societies.
AB - Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROα, MCP-1, RANTES, MIP-1α and MIP-1β expression was evaluated by flow cytometric analysis and RT-PCR in chondrocytes isolated from cartilage specimens obtained from patients with osteoarthritis and rheumatoid arthritis and multiorgan donors as normal controls. All the chemokines except RANTES were found in normal chondrocytes, with different degrees of staining intensity. In osteoarthritis and rheumatoid arthritis patients, an enhancement of IL-8, GROα, MIP-1α and MIP-1β was observed. Copyright (C) 1999 Federation of European Biochemical Societies.
KW - Chemokine
KW - Chondrocyte
KW - Flow cytometry
KW - Osteoarthritis
KW - Rheumatoid arthritis
UR - http://www.scopus.com/inward/record.url?scp=0032792867&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032792867&partnerID=8YFLogxK
U2 - 10.1016/S0014-5793(99)00886-8
DO - 10.1016/S0014-5793(99)00886-8
M3 - Article
C2 - 10437780
AN - SCOPUS:0032792867
VL - 455
SP - 238
EP - 242
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -