Flow cytometric analysis of intracellular chemokines in chondrocytes in vivo: Constitutive expression and enhancement in osteoarthritis and rheumatoid arthritis

Rosa Maria Borzì, Ilaria Mazzetti, Sandro Macor, Tania Silvestri, Alessandra Bassi, Luca Cattini, Andrea Facchini

Research output: Contribution to journalArticle

Abstract

Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROα, MCP-1, RANTES, MIP-1α and MIP-1β expression was evaluated by flow cytometric analysis and RT-PCR in chondrocytes isolated from cartilage specimens obtained from patients with osteoarthritis and rheumatoid arthritis and multiorgan donors as normal controls. All the chemokines except RANTES were found in normal chondrocytes, with different degrees of staining intensity. In osteoarthritis and rheumatoid arthritis patients, an enhancement of IL-8, GROα, MIP-1α and MIP-1β was observed. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)238-242
Number of pages5
JournalFEBS Letters
Volume455
Issue number3
DOIs
Publication statusPublished - Jul 23 1999

Keywords

  • Chemokine
  • Chondrocyte
  • Flow cytometry
  • Osteoarthritis
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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