Flow cytometric analysis of paraffin-embedded material in human gastric cancer

M. Danova, A. Riccardi, G. Mazzini, G. Wilson, P. Dionigi, S. Brugnatelli, R. Fiocca, G. Ucci, V. Jemos, E. Ascari

Research output: Contribution to journalArticlepeer-review

Abstract

Flow cytometric DNA analysis was performed on formalin-fixed, paraffin-embedded samples obtained by gastroscopic biopsy from 9 patients with histologically normal gastric mucosa (36 specimens) and by radical gastrectomy from 42 cases of human gastric cancer (120 specimens). Ploidy patterns and the distribution of cells in the different cell cycle phases were estimated, and the results were correlated with the histologic and clinical features. All samples of normal mucosa showed a diploid modal DNA content whereas DNA aneuploidy was encountered in 71.4% of the gastric tumors. The correlation between aneuploidy and histologic malignancy grading was statistically significant: aneuploidy was found in 36.4% of highly differentiated (grade 1 and grade 2) tumors and in 75.0% of poorly differentiated (grade 3) tumors (P

Original languageEnglish
Pages (from-to)200-206
Number of pages7
JournalAnalytical and Quantitative Cytology and Histology
Volume10
Issue number3
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy
  • Histology

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