Abstract
Using quantitative fluorescence in situ hybridisation and flow cytometry (flow-FISH), we investigated the biological and clinical relevance of telomere length in 55 patients affected by myelodysplastic syndromes (MDS) compared with 55 sex- and age-matched controls. We found that telomere fluorescence in MDS granulocytes, and CD34+ cells did not decline with age as in normal controls and that MDS granulocytes and CD34+ cells had significantly shorter telomeres than healthy controls. A significant higher incidence of cases with intermediate-unfavourable cytogenetics and International Prognostic Scoring System (IPSS) int-2/high-risk group was observed among patients with lower telomere fluorescence. We also found that apoptosis in CD34+ cells was significantly higher in IPSS int-1 low-risk patients when compared with IPSS int-2 high-risk cases and healthy controls and that CD34+ cell telomere fluorescence directly correlated with CD34+ cell apoptosis. Reduced telomere fluorescence was associated with a history of occupational exposure to toxic agents and with worse survival in univariate and multivariate analyses. Our results suggest that flow-cytometry assessment of telomere dynamics may represent a valuable tool in the biological and clinical-prognostic characterisation of MDS disorders.
| Original language | English |
|---|---|
| Pages (from-to) | 351-358 |
| Number of pages | 8 |
| Journal | European Journal of Haematology |
| Volume | 73 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - Nov 2004 |
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Keywords
- Flow cytometry
- Genotoxic agents
- Myelodysplastic syndromes
- Survival
- Telomere length
ASJC Scopus subject areas
- Hematology
Cite this
Flow cytometric detection of accelerated telomere shortening in myelodysplastic syndromes : Correlations with aetiological and clinical-biological findings. / Rigolin, Gian Matteo; Della Porta, Matteo; Bugli, Anna Maria; Castapari, Barbara; Mauro, Endri; Bragotti, Letiria Zenone; Ciccone, Maria; Cuneo, Antonio; Castoldi, Gianluigi.
In: European Journal of Haematology, Vol. 73, No. 5, 11.2004, p. 351-358.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Flow cytometric detection of accelerated telomere shortening in myelodysplastic syndromes
T2 - Correlations with aetiological and clinical-biological findings
AU - Rigolin, Gian Matteo
AU - Della Porta, Matteo
AU - Bugli, Anna Maria
AU - Castapari, Barbara
AU - Mauro, Endri
AU - Bragotti, Letiria Zenone
AU - Ciccone, Maria
AU - Cuneo, Antonio
AU - Castoldi, Gianluigi
PY - 2004/11
Y1 - 2004/11
N2 - Using quantitative fluorescence in situ hybridisation and flow cytometry (flow-FISH), we investigated the biological and clinical relevance of telomere length in 55 patients affected by myelodysplastic syndromes (MDS) compared with 55 sex- and age-matched controls. We found that telomere fluorescence in MDS granulocytes, and CD34+ cells did not decline with age as in normal controls and that MDS granulocytes and CD34+ cells had significantly shorter telomeres than healthy controls. A significant higher incidence of cases with intermediate-unfavourable cytogenetics and International Prognostic Scoring System (IPSS) int-2/high-risk group was observed among patients with lower telomere fluorescence. We also found that apoptosis in CD34+ cells was significantly higher in IPSS int-1 low-risk patients when compared with IPSS int-2 high-risk cases and healthy controls and that CD34+ cell telomere fluorescence directly correlated with CD34+ cell apoptosis. Reduced telomere fluorescence was associated with a history of occupational exposure to toxic agents and with worse survival in univariate and multivariate analyses. Our results suggest that flow-cytometry assessment of telomere dynamics may represent a valuable tool in the biological and clinical-prognostic characterisation of MDS disorders.
AB - Using quantitative fluorescence in situ hybridisation and flow cytometry (flow-FISH), we investigated the biological and clinical relevance of telomere length in 55 patients affected by myelodysplastic syndromes (MDS) compared with 55 sex- and age-matched controls. We found that telomere fluorescence in MDS granulocytes, and CD34+ cells did not decline with age as in normal controls and that MDS granulocytes and CD34+ cells had significantly shorter telomeres than healthy controls. A significant higher incidence of cases with intermediate-unfavourable cytogenetics and International Prognostic Scoring System (IPSS) int-2/high-risk group was observed among patients with lower telomere fluorescence. We also found that apoptosis in CD34+ cells was significantly higher in IPSS int-1 low-risk patients when compared with IPSS int-2 high-risk cases and healthy controls and that CD34+ cell telomere fluorescence directly correlated with CD34+ cell apoptosis. Reduced telomere fluorescence was associated with a history of occupational exposure to toxic agents and with worse survival in univariate and multivariate analyses. Our results suggest that flow-cytometry assessment of telomere dynamics may represent a valuable tool in the biological and clinical-prognostic characterisation of MDS disorders.
KW - Flow cytometry
KW - Genotoxic agents
KW - Myelodysplastic syndromes
KW - Survival
KW - Telomere length
UR - http://www.scopus.com/inward/record.url?scp=5344279030&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=5344279030&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0609.2004.00305.x
DO - 10.1111/j.1600-0609.2004.00305.x
M3 - Article
C2 - 15458514
AN - SCOPUS:5344279030
VL - 73
SP - 351
EP - 358
JO - European Journal of Haematology
JF - European Journal of Haematology
SN - 0902-4441
IS - 5
ER -