Flow cytometric detection of accelerated telomere shortening in myelodysplastic syndromes: Correlations with aetiological and clinical-biological findings

Gian Matteo Rigolin, Matteo Della Porta, Anna Maria Bugli, Barbara Castapari, Endri Mauro, Letiria Zenone Bragotti, Maria Ciccone, Antonio Cuneo, Gianluigi Castoldi

Research output: Contribution to journalArticle

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Abstract

Using quantitative fluorescence in situ hybridisation and flow cytometry (flow-FISH), we investigated the biological and clinical relevance of telomere length in 55 patients affected by myelodysplastic syndromes (MDS) compared with 55 sex- and age-matched controls. We found that telomere fluorescence in MDS granulocytes, and CD34+ cells did not decline with age as in normal controls and that MDS granulocytes and CD34+ cells had significantly shorter telomeres than healthy controls. A significant higher incidence of cases with intermediate-unfavourable cytogenetics and International Prognostic Scoring System (IPSS) int-2/high-risk group was observed among patients with lower telomere fluorescence. We also found that apoptosis in CD34+ cells was significantly higher in IPSS int-1 low-risk patients when compared with IPSS int-2 high-risk cases and healthy controls and that CD34+ cell telomere fluorescence directly correlated with CD34+ cell apoptosis. Reduced telomere fluorescence was associated with a history of occupational exposure to toxic agents and with worse survival in univariate and multivariate analyses. Our results suggest that flow-cytometry assessment of telomere dynamics may represent a valuable tool in the biological and clinical-prognostic characterisation of MDS disorders.

Original languageEnglish
Pages (from-to)351-358
Number of pages8
JournalEuropean Journal of Haematology
Volume73
Issue number5
DOIs
Publication statusPublished - Nov 2004

Fingerprint

Telomere Shortening
Myelodysplastic Syndromes
Telomere
Fluorescence
Granulocytes
Flow Cytometry
Apoptosis
Poisons
Occupational Exposure
Fluorescence In Situ Hybridization
Cytogenetics
Multivariate Analysis
Survival
Incidence

Keywords

  • Flow cytometry
  • Genotoxic agents
  • Myelodysplastic syndromes
  • Survival
  • Telomere length

ASJC Scopus subject areas

  • Hematology

Cite this

Flow cytometric detection of accelerated telomere shortening in myelodysplastic syndromes : Correlations with aetiological and clinical-biological findings. / Rigolin, Gian Matteo; Della Porta, Matteo; Bugli, Anna Maria; Castapari, Barbara; Mauro, Endri; Bragotti, Letiria Zenone; Ciccone, Maria; Cuneo, Antonio; Castoldi, Gianluigi.

In: European Journal of Haematology, Vol. 73, No. 5, 11.2004, p. 351-358.

Research output: Contribution to journalArticle

Rigolin, Gian Matteo ; Della Porta, Matteo ; Bugli, Anna Maria ; Castapari, Barbara ; Mauro, Endri ; Bragotti, Letiria Zenone ; Ciccone, Maria ; Cuneo, Antonio ; Castoldi, Gianluigi. / Flow cytometric detection of accelerated telomere shortening in myelodysplastic syndromes : Correlations with aetiological and clinical-biological findings. In: European Journal of Haematology. 2004 ; Vol. 73, No. 5. pp. 351-358.
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