Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects

S. Mariucci, B. Rovati, K. Bencardino, M. Manzoni, M. Danova

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Circulating endothelial cells (CEC) and endothelial progenitor cells (CEP) play an important role in tissue neovascularization. In human tumours, these cells may have clinical implications as prognostic/ predictive factors during antiangiogenic therapy. The lack of a standardized assay for the quantification of these rare events has lead to a wide variation in the reported ranges of CEC and CEP. This study aimedtodevelop aflow cytometric (FCM) methodfor theimmunophenotipic detection and enumeration of these cells in a healthy population. Peripheral blood samples from 32 subjects were analysed. Multiparameter FCM analysis was used to quantify resting and activated CEC and CEP. The mean values of the percentage and of the absolute number were: 0.005 ± 0.004% and 306 ± 243 cells/ml for CEC;0.002 ± 0.001% and 130 ± 110 cells/ml for rCEC;0.003 ± 0.002% and 176 ± 150 cells/ml for aCEC; 0.0001 ± 0.00005% and 6 ± 2 for CEP. We confirmed that FCM is an accurate and sensitive method for the quantitative analysis of CEC and CEP. The determination of normal ranges of CEC and CEP is helpful in defining their role as surrogate biomarkers of antiangiogenic treatment efficacy during clinical trials in oncology.

Original languageEnglish
JournalInternational Journal of Laboratory Hematology
Volume32
Issue number1 PART.1
DOIs
Publication statusPublished - Feb 2010

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Endothelial cells
Healthy Volunteers
Endothelial Cells
Endothelial Progenitor Cells
Reference Values
Biomarkers
Clinical Trials
Oncology
Population
Tumors
Assays
Neoplasms
Blood
Tissue

Keywords

  • Angiogenesis
  • Circulating endothelial cells
  • Endothelial progenitors
  • Flow cytometry
  • Normal reference values

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Hematology

Cite this

Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. / Mariucci, S.; Rovati, B.; Bencardino, K.; Manzoni, M.; Danova, M.

In: International Journal of Laboratory Hematology, Vol. 32, No. 1 PART.1, 02.2010.

Research output: Contribution to journalArticle

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