Il contenuto di DNA flussocitometrico è correlato alla ricaduta di malattia in bambini con neuroblastoma

Translated title of the contribution: Flow cytometric DNA content helps predicting recurrence in children with neuroblastoma

E. Geido, C. Peveri, E. Infusini, E. Zeraschi, A. Di Vinci, W. Giaretti, C. Milanaccio, G. P. Tonini, B. De Bernardi, C. Lo Cunsolo, P. Alvisi, A. Pession, M. Lastilla

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aim of the study was to evaluate the prognostic value of DNA index (DI), S-phase fraction (SPF) and N-myc amplification in children with neuroblastoma using fresh or frozen samples obtained at diagnosis. The study includes 123 children with a mean age of 26 months followed-up for a mean time of 37 months to evaluate the overall survival (OS) and the event-free survival (EFS). Death and recurrence incidences were respectively 15% (18 cases) and 25% (30 cases). DNA aneuploidy (DI ≠ 1) incidence was 85/123 (69%) while median SPF was 6.1%. N-myc amplification was detected in 17/119 cases (14%) of which 15 corresponded to stage 4 tumors. Univariate analysis showed that OS probability was significantly correlated with stage (p = 0.0001), age (p = 0.0002), DI (p = 0.002) and, at lesser degree, with N-myc (p = 0.04). In particular, for the subgroup of children with stage 4, OS was only slightly influenced by DI (p = 0.02). Univariate probability EFS for the prediction of recurrence (excluding stage 4s cases) was found to be high in children with tumours having DNA diploid (p = 0.001) and stage 4 (p = 0.001). SPF values were not useful to predict neither OS nor EFS. Multivariate analysis was performed for both OS and EFS data: stage and age but not DI were relevant (p <0.01) for predicting OS, while DI and stage but not age were relevant for predicting recurrence (EFS). In conclusion, the present study indicates that flow cytometric DNA diploidy is an important prognostic factor of recurrence in children with neuroblastoma.

Original languageItalian
Pages (from-to)912-919
Number of pages8
JournalRivista Italiana di Pediatria
Volume22
Issue number6
Publication statusPublished - 1996

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Neuroblastoma
Recurrence
DNA
Disease-Free Survival
S Phase
Diploidy
Incidence
Aneuploidy
Neoplasms
Multivariate Analysis

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Geido, E., Peveri, C., Infusini, E., Zeraschi, E., Di Vinci, A., Giaretti, W., ... Lastilla, M. (1996). Il contenuto di DNA flussocitometrico è correlato alla ricaduta di malattia in bambini con neuroblastoma. Rivista Italiana di Pediatria, 22(6), 912-919.

Il contenuto di DNA flussocitometrico è correlato alla ricaduta di malattia in bambini con neuroblastoma. / Geido, E.; Peveri, C.; Infusini, E.; Zeraschi, E.; Di Vinci, A.; Giaretti, W.; Milanaccio, C.; Tonini, G. P.; De Bernardi, B.; Lo Cunsolo, C.; Alvisi, P.; Pession, A.; Lastilla, M.

In: Rivista Italiana di Pediatria, Vol. 22, No. 6, 1996, p. 912-919.

Research output: Contribution to journalArticle

Geido, E, Peveri, C, Infusini, E, Zeraschi, E, Di Vinci, A, Giaretti, W, Milanaccio, C, Tonini, GP, De Bernardi, B, Lo Cunsolo, C, Alvisi, P, Pession, A & Lastilla, M 1996, 'Il contenuto di DNA flussocitometrico è correlato alla ricaduta di malattia in bambini con neuroblastoma', Rivista Italiana di Pediatria, vol. 22, no. 6, pp. 912-919.
Geido E, Peveri C, Infusini E, Zeraschi E, Di Vinci A, Giaretti W et al. Il contenuto di DNA flussocitometrico è correlato alla ricaduta di malattia in bambini con neuroblastoma. Rivista Italiana di Pediatria. 1996;22(6):912-919.
Geido, E. ; Peveri, C. ; Infusini, E. ; Zeraschi, E. ; Di Vinci, A. ; Giaretti, W. ; Milanaccio, C. ; Tonini, G. P. ; De Bernardi, B. ; Lo Cunsolo, C. ; Alvisi, P. ; Pession, A. ; Lastilla, M. / Il contenuto di DNA flussocitometrico è correlato alla ricaduta di malattia in bambini con neuroblastoma. In: Rivista Italiana di Pediatria. 1996 ; Vol. 22, No. 6. pp. 912-919.
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abstract = "Aim of the study was to evaluate the prognostic value of DNA index (DI), S-phase fraction (SPF) and N-myc amplification in children with neuroblastoma using fresh or frozen samples obtained at diagnosis. The study includes 123 children with a mean age of 26 months followed-up for a mean time of 37 months to evaluate the overall survival (OS) and the event-free survival (EFS). Death and recurrence incidences were respectively 15{\%} (18 cases) and 25{\%} (30 cases). DNA aneuploidy (DI ≠ 1) incidence was 85/123 (69{\%}) while median SPF was 6.1{\%}. N-myc amplification was detected in 17/119 cases (14{\%}) of which 15 corresponded to stage 4 tumors. Univariate analysis showed that OS probability was significantly correlated with stage (p = 0.0001), age (p = 0.0002), DI (p = 0.002) and, at lesser degree, with N-myc (p = 0.04). In particular, for the subgroup of children with stage 4, OS was only slightly influenced by DI (p = 0.02). Univariate probability EFS for the prediction of recurrence (excluding stage 4s cases) was found to be high in children with tumours having DNA diploid (p = 0.001) and stage 4 (p = 0.001). SPF values were not useful to predict neither OS nor EFS. Multivariate analysis was performed for both OS and EFS data: stage and age but not DI were relevant (p <0.01) for predicting OS, while DI and stage but not age were relevant for predicting recurrence (EFS). In conclusion, the present study indicates that flow cytometric DNA diploidy is an important prognostic factor of recurrence in children with neuroblastoma.",
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AU - Peveri, C.

AU - Infusini, E.

AU - Zeraschi, E.

AU - Di Vinci, A.

AU - Giaretti, W.

AU - Milanaccio, C.

AU - Tonini, G. P.

AU - De Bernardi, B.

AU - Lo Cunsolo, C.

AU - Alvisi, P.

AU - Pession, A.

AU - Lastilla, M.

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N2 - Aim of the study was to evaluate the prognostic value of DNA index (DI), S-phase fraction (SPF) and N-myc amplification in children with neuroblastoma using fresh or frozen samples obtained at diagnosis. The study includes 123 children with a mean age of 26 months followed-up for a mean time of 37 months to evaluate the overall survival (OS) and the event-free survival (EFS). Death and recurrence incidences were respectively 15% (18 cases) and 25% (30 cases). DNA aneuploidy (DI ≠ 1) incidence was 85/123 (69%) while median SPF was 6.1%. N-myc amplification was detected in 17/119 cases (14%) of which 15 corresponded to stage 4 tumors. Univariate analysis showed that OS probability was significantly correlated with stage (p = 0.0001), age (p = 0.0002), DI (p = 0.002) and, at lesser degree, with N-myc (p = 0.04). In particular, for the subgroup of children with stage 4, OS was only slightly influenced by DI (p = 0.02). Univariate probability EFS for the prediction of recurrence (excluding stage 4s cases) was found to be high in children with tumours having DNA diploid (p = 0.001) and stage 4 (p = 0.001). SPF values were not useful to predict neither OS nor EFS. Multivariate analysis was performed for both OS and EFS data: stage and age but not DI were relevant (p <0.01) for predicting OS, while DI and stage but not age were relevant for predicting recurrence (EFS). In conclusion, the present study indicates that flow cytometric DNA diploidy is an important prognostic factor of recurrence in children with neuroblastoma.

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