TY - JOUR
T1 - Flow cytometric DNA ploidy, p53, PCNA, and c-erbB-2 protein expressions as predictors of survival in surgically resected gastric cancer patients
AU - Danesi, Donatella Tirindelli
AU - Spanò, Marcello
AU - Fabiano, Alfredo
AU - Altavista, Pierluigi
AU - Pasqualetti, Patrizio
AU - Toscano, Maria Gabriella
AU - Antonini, Fabiana
AU - Catalano, Piera
AU - Mecozzi, Antonella
AU - Picconi, Antonello
AU - Daffinà, Antonio
AU - Cucchiara, Giuseppe
PY - 2000/2/15
Y1 - 2000/2/15
N2 - In order to determine retrospectively the impact of some cytometric and immunohistochemical parameters on the overall survival of gastric cancer patients treated with surgery alone, paraffin-embedded tumor samples from 137 gastric carcinoma patients undergoing curative resection from 1987-1993 were analyzed by flow cytometry (FCM) and immunohistochemistry (p53, c-erbB- 2, and PCNA expression). FCM-derived parameters were DNA ploidy and fraction of S-phase cells (SPF). Multiple regression analysis was applied to determine the prognostic significance of the conventional clinicopathologic findings together with the flow cytometric and immunohistochemical parameters on overall survival. When all parameters were entered simultaneously into the Cox regression model, stage and DNA ploidy (DNA index > 1.35) clearly emerged as the only independent prognostic factors. When the stages were analysed separately, the independent prognostic factors resulted DNA ploidy in early stages (I-II) and grading in stage IIIA tumors. For stage IIIB tumors, no independent prognostic factor was found. These results indicate that the DNA ploidy pattern is a valuable predictor of survival in curatively resected gastric cancer patients, especially when less advanced tumors are taken into consideration. (C) 2000 Wiley-Liss, Inc.
AB - In order to determine retrospectively the impact of some cytometric and immunohistochemical parameters on the overall survival of gastric cancer patients treated with surgery alone, paraffin-embedded tumor samples from 137 gastric carcinoma patients undergoing curative resection from 1987-1993 were analyzed by flow cytometry (FCM) and immunohistochemistry (p53, c-erbB- 2, and PCNA expression). FCM-derived parameters were DNA ploidy and fraction of S-phase cells (SPF). Multiple regression analysis was applied to determine the prognostic significance of the conventional clinicopathologic findings together with the flow cytometric and immunohistochemical parameters on overall survival. When all parameters were entered simultaneously into the Cox regression model, stage and DNA ploidy (DNA index > 1.35) clearly emerged as the only independent prognostic factors. When the stages were analysed separately, the independent prognostic factors resulted DNA ploidy in early stages (I-II) and grading in stage IIIA tumors. For stage IIIB tumors, no independent prognostic factor was found. These results indicate that the DNA ploidy pattern is a valuable predictor of survival in curatively resected gastric cancer patients, especially when less advanced tumors are taken into consideration. (C) 2000 Wiley-Liss, Inc.
KW - c-erbB-2
KW - DNA flow cytometry
KW - Gastric cancer
KW - p53
KW - PCNA
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U2 - 10.1002/(SICI)1097-0320(20000215)42:1<27::AID-CYTO5>3.0.CO;2-N
DO - 10.1002/(SICI)1097-0320(20000215)42:1<27::AID-CYTO5>3.0.CO;2-N
M3 - Article
C2 - 10679740
AN - SCOPUS:0034652606
VL - 42
SP - 27
EP - 34
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
SN - 1552-4949
IS - 1
ER -