Flow cytometry analysis and crossmatch detection techniques in transplantation

Vincenzo Grimaldi, Elena Cesario, Amelia Casamassimi, Teresa Infante, Claudio Napoli

Research output: Contribution to journalArticle

Abstract

The relevance of anti-human leucocyte antigen (anti-HLA) antibodies in the outcome of organ transplantation has been recognized in the past 40 years. Detection of these antibodies in sensitized recipients plays a pivotal role in this issue. Flow cytometry analysis is one of the choice cell-based techniques together with the pioneer complementdependent cytotoxicity crossmatch. Here, we discuss the advantages and limits of these cell-based assays and their use in the clinical laboratory of immunology of transplantation. Moreover, we compared this approach to other beads-based detection assays introduced more recently in the laboratory practice.

Original languageEnglish
Pages (from-to)34-39
Number of pages6
JournalImmunology, Endocrine and Metabolic Agents in Medicinal Chemistry
Volume12
Issue number1
Publication statusPublished - Mar 2012

Keywords

  • Anti-HLA antibodies
  • Beads-based assay
  • Cell-based assay
  • Complement-dependent cytotoxicity crossmatch
  • Flow cytometry crossmatch
  • Luminex crossmatch
  • Luminex single antigen technology
  • Monoclonal antibodies
  • Organ transplantation
  • Solid-phase assay
  • Virtual crossmatch
  • XM-ONE

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Immunology and Allergy
  • Pharmacology

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