Flow Cytometry Assessment of CD26+ Leukemic Stem Cells in Peripheral Blood: A Simple and Rapid New Diagnostic Tool for Chronic Myeloid Leukemia

Donatella Raspadori, Paola Pacelli, Anna Sicuranza, Elisabetta Abruzzese, Alessandra Iurlo, Daniele Cattaneo, Antonella Gozzini, Sara Galimberti, Claudia Baratè, Patrizia Pregno, Maura Nicolosi, Federica Sorà, Mario Annunziata, Luigiana Luciano, Giovanni Caocci, Sabrina Moretti, Nicola Sgherza, Claudio Fozza, Sabina Russo, Emilio UsalaMarina A. Liberati, Sara Ciofini, Monika M. Trawinska, Alessandro Gozzetti, Monica Bocchia

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Recent investigations in chronic myeloid leukemia (CML) have focused on the identification and characterization of leukemic stem cells (LSCs). These cells reside within the CD34+/CD38/Lin fraction and score positive for CD26 (dipeptidylpeptidase IV) a marker, expressed in both bone marrow (BM) and peripheral blood (PB) samples, that discriminates CML cells from normal hematopoietic stem cells (HSCs) or from LSCs of other myeloid neoplasms. CD26 evaluation could be a useful tool to improve the identification of CML LCSs by using flow-cytometry assay. Methods: CD26+ LSCs have been isolated from EDTA PB and BM samples of patients with leucocytosis suspected for CML. Analysis of LSCs CML has been performed by using custom-made lyophilized pre-titrated antibody mixture test and control tube and a CD45+/CD34+/CD38/CD26+ panel as a strict flow cytometric gating strategy. Results: The expression of CD26 on CD34+/CD38 population was detectable in 211/211 PB and 84/84 BM samples of subsequently confirmed BCR-ABL+ CP-CML patients. None of the 32 samples suspicious for CML but scoring negative for circulating CD26+ LSCs were diagnosed as CML after conventional cytogenetic and molecular testing. To validate our results, we checked for PB CD26+ LSCs in patients affected by other hematological disorders and they all scored negative for CD26 expression. Conclusions: We propose flow cytometry evaluation of CD26 expression on PB CD34+/CD38 population as a new rapid, reproducible, and powerful diagnostic tool for the diagnosis of CML.

Original languageEnglish
Pages (from-to)294-299
Number of pages6
JournalCytometry Part B - Clinical Cytometry
Volume96
Issue number4
DOIs
Publication statusPublished - Jul 2019

Keywords

  • CD26
  • chronic myeloid leukemia
  • diagnosis
  • flow cytometry
  • leukemic stem cells
  • peripheral blood

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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