Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets

Ornella Bonavita, Matteo Massara, Achille Anselmo, Paolo Somma, Hilke Brühl, Matthias MacK, Massimo Locati, Raffaella Bonecchi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chemokine receptors are differentially expressed on leukocyte subpopulations dictating their ability to migrate both in physiological and pathological conditions. Their expression is modulated during leukocyte differentiation and maturation and they can be used as markers to identify and characterize the frequency and the activation state of leukocytes present in a tissue. Here, we will describe flow cytometry approaches to detect chemokine receptors identifying subpopulations of circulating monocytes and neutrophils.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages441-456
Number of pages16
Volume570
DOIs
Publication statusPublished - 2016

Publication series

NameMethods in Enzymology
Volume570
ISSN (Print)00766879
ISSN (Electronic)15577988

Keywords

  • Chemokine receptors
  • Chemokines
  • Flow cytometry
  • Monocytes
  • Neutrophils

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Flow Cytometry Detection of Chemokine Receptors for the Identification of Murine Monocyte and Neutrophil Subsets'. Together they form a unique fingerprint.

Cite this