Flow cytometry evaluation of erythroid dysplasia in patients with myelodysplastic syndrome

M. G. Della Porta, L. Malcovati, R. Invernizzi, E. Travaglino, C. Pascutto, M. Maffioli, A. Gallí, S. Boggi, D. Pietra, L. Vanelli, C. Marseglia, S. Levi, P. Arosio, M. Lazzarino, Mario Cazzola

Research output: Contribution to journalArticle

Abstract

Erythroid dysplasia is the pathologic hallmark of myelodysplastic syndromes (MDS). To develop a quantitative flow-cytometry approach to its evaluation, we analyzed the expression of CD71, CD105, cytosolic H-ferritin (HF), cytosolic L-ferritin (LF) and mitochondrial ferritin (MtF) in erythroblasts from 104 MDS patients, 69 pathologic control patients and 19 healthy subjects. Six-parameter, 4-color flow cytometry was employed, and data were expressed as mean fluorescence intensity. Compared with pathologic and healthy controls, MDS patients had higher expression of HF (P95% of MDS patients. This flow-cytometry approach provides an accurate quantitative evaluation of erythroid dysplasia and allows a reliable diagnosis of sideroblastic anemia, and may therefore be a useful tool in the work-up of patients with MDS.

Original languageEnglish
Pages (from-to)549-555
Number of pages7
JournalLeukemia
Volume20
Issue number4
DOIs
Publication statusPublished - Apr 2006

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Keywords

  • Erythroid dysplasia
  • Ferritin
  • Flow cytometry
  • Immunophenotyping
  • Myelodysplastic syndrome
  • Transferrin receptor

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Della Porta, M. G., Malcovati, L., Invernizzi, R., Travaglino, E., Pascutto, C., Maffioli, M., Gallí, A., Boggi, S., Pietra, D., Vanelli, L., Marseglia, C., Levi, S., Arosio, P., Lazzarino, M., & Cazzola, M. (2006). Flow cytometry evaluation of erythroid dysplasia in patients with myelodysplastic syndrome. Leukemia, 20(4), 549-555. https://doi.org/10.1038/sj.leu.2404142