Flow cytometry in situ polymerase chain reaction as an innovative approach to HIV-1 proviral DNA detection

D. Gibellini, G. Zauli, M. C. Re, G. Furlini, M. La Placa

Research output: Contribution to journalArticle

Abstract

In situ polymerase chain reaction represents an intriguing method to couple the sensitivity of PCR technology with the preservation of cell morphology. The target of this technique is the detection of specific DNA or retrotranscribed RNA inside the cell. In this brief report, we describe an in situ polymerase chain reaction technique revealed by flow cytometry in order to reveal the human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells. We also discuss the key steps and parameters of our assay in comparison with current opinion on in situ polymerase chain reaction.

Original languageEnglish
Pages (from-to)137-141
Number of pages5
JournalEuropean journal of histochemistry : EJH
Volume42
Issue number2
Publication statusPublished - 1998

Keywords

  • Flow cytometry
  • HIV-1
  • In situ PCR
  • PBMC

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Cell Biology
  • Developmental Biology
  • Anatomy

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