Flow cytometry vs cytomorphology for the detection of hematologic malignancy in body cavity fluids

Clara Cesana, Catherine Klersy, Barbara Scarpati, Bruno Brando, Elisabetta Volpato, Giambattista Bertani, Maurizio Faleri, Annamaria Nosari, Silvia Cantoni, Ursula Ferri, Linda Scampini, Claudia Barba, Giuliana Lando, Enrica Morra, Roberto Cairoli

Research output: Contribution to journalArticlepeer-review

Abstract

Flow cytometry and cytomorphology results on 92 body cavity fluids [61 effusions and 31 bronchoalveolar lavage fluids (BALF)] from hematologic malignancy were compared with retrospective clinical outcome. We observed double true positive/negative results in 67 cases (73%), and double false negative results in 2 cases (2%). Immunophenotyping accounted for true positive/negative results in 22 out of 23 mismatched cases (25%), and retained significantly higher accuracy than that of cytomorphology especially in effusions and differentiated lymphoma. In BALF analysis, immunophenotyping and cytomorphology sensitivity was 75% and 0%, respectively. Flow cytometry retains the highest accuracy in detecting neoplastic cells in body cavity fluids.

Original languageEnglish
Pages (from-to)1027-1034
Number of pages8
JournalLeukemia Research
Volume34
Issue number8
DOIs
Publication statusPublished - Aug 2010

Keywords

  • Bronchoalveolar lavage fluid
  • Cytomorphology
  • Flow cytometry
  • Hematologic malignancy
  • Non-Hodgkin lymphoma
  • Serous effusion

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

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