Fluorescence and energy transfer of tryptophans in Aplysia myoglobin.

S. M. Janes, G. Holtom, P. Ascenzi, M. Brunori, R. M. Hochstrasser

Research output: Contribution to journalArticle

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Abstract

The fluorescence decay of tryptophan residues in apo and met Aplysia limacina myoglobin and sperm whale myoglobin were measured in aqueous solution at 10 degrees-15 degrees C. In all species, multiexponential behavior was observed in which the individual components displayed unique frequency-dependent emission characteristics. The results suggest that the tryptophan fluorescence in all met samples are quenched by rapid Forster energy transfer to the heme as predicted from the crystal geometry. Fluorescence from the apo protein is similar to that in solutions of free tryptophans. In addition, the fluorescence properties of the reversible thermal denaturation of Aplysia limacina met myoglobin was investigated between 25 degrees and 75 degrees C.

Original languageEnglish
Pages (from-to)653-660
Number of pages8
JournalBiophysical Journal
Volume51
Issue number4
Publication statusPublished - Apr 1987

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Aplysia
Myoglobin
Energy Transfer
Tryptophan
Fluorescence
Sperm Whale
Metmyoglobin
Heme
Hot Temperature
Proteins

ASJC Scopus subject areas

  • Biophysics

Cite this

Janes, S. M., Holtom, G., Ascenzi, P., Brunori, M., & Hochstrasser, R. M. (1987). Fluorescence and energy transfer of tryptophans in Aplysia myoglobin. Biophysical Journal, 51(4), 653-660.

Fluorescence and energy transfer of tryptophans in Aplysia myoglobin. / Janes, S. M.; Holtom, G.; Ascenzi, P.; Brunori, M.; Hochstrasser, R. M.

In: Biophysical Journal, Vol. 51, No. 4, 04.1987, p. 653-660.

Research output: Contribution to journalArticle

Janes, SM, Holtom, G, Ascenzi, P, Brunori, M & Hochstrasser, RM 1987, 'Fluorescence and energy transfer of tryptophans in Aplysia myoglobin.', Biophysical Journal, vol. 51, no. 4, pp. 653-660.
Janes SM, Holtom G, Ascenzi P, Brunori M, Hochstrasser RM. Fluorescence and energy transfer of tryptophans in Aplysia myoglobin. Biophysical Journal. 1987 Apr;51(4):653-660.
Janes, S. M. ; Holtom, G. ; Ascenzi, P. ; Brunori, M. ; Hochstrasser, R. M. / Fluorescence and energy transfer of tryptophans in Aplysia myoglobin. In: Biophysical Journal. 1987 ; Vol. 51, No. 4. pp. 653-660.
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