Fluorescence anisotropy analysis of protein-antibody interaction

Nadia Barbero, Lucia Napione, Pierluigi Quagliotto, Simona Pavan, Claudia Barolo, Ermanno Barni, Federico Bussolino, Guido Viscardi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The interaction between glutathione S-transferase and its antibody α-glutathione S-transferase (B-14) was studied using fluorescence anisotropy, subsequent to glutathione S-transferase bioconjugation with fluorescein-5-maleimide, leading to the determination of the dissociation and association binding constants, Kd and Ka; good binding specificity was observed between glutathione S-transferase and the antibody B-14. The use of spectroscopic techniques, fluorescence anisotropy in particular, is a useful and favourable tool to study biochemical problems.

Original languageEnglish
Pages (from-to)225-229
Number of pages5
JournalDyes and Pigments
Volume83
Issue number2
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Glutathione Transferase
Antibodies
Anisotropy
Fluorescence
Proteins
Association reactions
Glutathione
desintegron B

Keywords

  • Bioconjugation
  • Fluorescein-5-maleimide
  • Fluorescence anisotropy
  • Glutathione S-transferase (GST)
  • Protein-antibody interaction

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Process Chemistry and Technology

Cite this

Barbero, N., Napione, L., Quagliotto, P., Pavan, S., Barolo, C., Barni, E., ... Viscardi, G. (2009). Fluorescence anisotropy analysis of protein-antibody interaction. Dyes and Pigments, 83(2), 225-229. https://doi.org/10.1016/j.dyepig.2009.04.011

Fluorescence anisotropy analysis of protein-antibody interaction. / Barbero, Nadia; Napione, Lucia; Quagliotto, Pierluigi; Pavan, Simona; Barolo, Claudia; Barni, Ermanno; Bussolino, Federico; Viscardi, Guido.

In: Dyes and Pigments, Vol. 83, No. 2, 11.2009, p. 225-229.

Research output: Contribution to journalArticle

Barbero, N, Napione, L, Quagliotto, P, Pavan, S, Barolo, C, Barni, E, Bussolino, F & Viscardi, G 2009, 'Fluorescence anisotropy analysis of protein-antibody interaction', Dyes and Pigments, vol. 83, no. 2, pp. 225-229. https://doi.org/10.1016/j.dyepig.2009.04.011
Barbero N, Napione L, Quagliotto P, Pavan S, Barolo C, Barni E et al. Fluorescence anisotropy analysis of protein-antibody interaction. Dyes and Pigments. 2009 Nov;83(2):225-229. https://doi.org/10.1016/j.dyepig.2009.04.011
Barbero, Nadia ; Napione, Lucia ; Quagliotto, Pierluigi ; Pavan, Simona ; Barolo, Claudia ; Barni, Ermanno ; Bussolino, Federico ; Viscardi, Guido. / Fluorescence anisotropy analysis of protein-antibody interaction. In: Dyes and Pigments. 2009 ; Vol. 83, No. 2. pp. 225-229.
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