Fluorescence polarization assay for endothelin-converting enzymes

S. Hazan, I. Fishov, M. Zamai, V. R. Caiolfa, A. H. Parola

Research output: Contribution to journalArticlepeer-review

Abstract

The conversion of big endothelin to endothelin by α-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.

Original languageEnglish
Pages (from-to)833-836
Number of pages4
JournalPeptides
Volume16
Issue number5
DOIs
Publication statusPublished - 1995

Keywords

  • Endothelin
  • Endothelin-converting enzyme assay
  • Fluorescence polarization
  • Tryptophan fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

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