Fluorescence polarization assay for endothelin-converting enzymes

S. Hazan, I. Fishov, M. Zamai, V. R. Caiolfa, A. H. Parola

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The conversion of big endothelin to endothelin by α-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.

Original languageEnglish
Pages (from-to)833-836
Number of pages4
JournalPeptides
Volume16
Issue number5
DOIs
Publication statusPublished - 1995

Fingerprint

Fluorescence Polarization
Endothelins
Chymotrypsin
Endothelin-1
Assays
Fluorescence
Polarization
Enzymes
Endothelin-Converting Enzymes

Keywords

  • Endothelin
  • Endothelin-converting enzyme assay
  • Fluorescence polarization
  • Tryptophan fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Fluorescence polarization assay for endothelin-converting enzymes. / Hazan, S.; Fishov, I.; Zamai, M.; Caiolfa, V. R.; Parola, A. H.

In: Peptides, Vol. 16, No. 5, 1995, p. 833-836.

Research output: Contribution to journalArticle

Hazan, S. ; Fishov, I. ; Zamai, M. ; Caiolfa, V. R. ; Parola, A. H. / Fluorescence polarization assay for endothelin-converting enzymes. In: Peptides. 1995 ; Vol. 16, No. 5. pp. 833-836.
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