The conversion of big endothelin to endothelin by α-chymotrypsin was determined by following its single Trp fluorescence polarization. This provides a novel, simple, fast, and sensitive identifying assay in the search for a native endothelin-converting enzyme.
- Endothelin-converting enzyme assay
- Fluorescence polarization
- Tryptophan fluorescence
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience