Fluorimetric methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, ß-hydroxybutyrate, glycerol) using a COBAS FAR A centrifugal analyser

L. D. Monti, P. E. Sandoli, S. Costa, V. C. Phan, P. M. Piatti

Research output: Contribution to journalArticle

Abstract

Intermediate products of the metabolism of glucose, fat and amino-acid are important in the evaluation of such metabolic disorders as diabetes mellitus, liver disease and metabolic acidosis. In the present study, methods for the measurement of intermediate metabolites (lactate, pyruvate, alanine, ß-hydroxybutyrate and glycerol) have been adapted to a fast centrifugal analyzer: the COBAS FARA. Correlation coefficients ranged from 0.90 to 0.99, compared to established manual spectrophotometric methods. Within-run coefficients of variation (CVs) ranged between 2.9 and 8.8% at low levels, between 1.5 and 5.7% at medium levels and between 1-2 and 5-6% at high levels. Between-run CVs were between 4-0 and 15.0%o at low levels, between 1.7 and 7-0%o at medium levels and between 1.3 and 2.7%0 at high levels. These fluorimetric assays for the determination of intermediate metabolites on COBAS FARA (Roche) have a good sensitivity and precision, are less costly than manual methods and can be used on a routine basis.

Original languageEnglish
Pages (from-to)177-181
Number of pages5
JournalJournal of Automatic Chemistry
Volume15
Issue number5
DOIs
Publication statusPublished - 1993

ASJC Scopus subject areas

  • Clinical Biochemistry

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