A high degree of reproducibility was obtained using a simple and rapid microfluorimetric method of DNA determination. The described method evaluates the increased fluorescence after reaction of 33258 Hoechst fluorochrome with calf thymus DNA and mouse liver chromatin preparations. The routinely used preparation reagents and the subcellular components do not interfere with the reaction.
|Translated title of the contribution||Fluorometric DNA assay at nanogram levels in biological material|
|Number of pages||7|
|Journal||Bollettino della Societa Italiana di Biologia Sperimentale|
|Publication status||Published - Aug 30 1980|
ASJC Scopus subject areas