Follicle-like model by granulosa cell encapsulation in a barium alginate-protamine membrane

D. Vigo, S. Villani, M. Faustini, P. A. Accorsi, G. Galeati, M. Spinaci, E. Munari, V. Russo, A. Asti, U. Conte, M. L. Torre

Research output: Contribution to journalArticlepeer-review


Granulosa cells from bovine and porcine ovaries were cultured either in monolayer or in follicle-like barium alginate capsules for 6 days. Morphological investigation by electron scanning microscopy indicated that culture in a three-dimensional (3D) system allows self-organization of spherical-polyhedral shape cells. The luteinization index (progesterone: 17β-estradiol ratio) was significantly higher for monolayer cells than for the 3D cell culture system, confirming the results of morphological analysis and indicating more physiological growth. The encapsulated 3D culture system appears to be a promising way of obtaining in vitro maturation and development of follicles and oocytes.

Original languageEnglish
Pages (from-to)709-714
Number of pages6
JournalTissue Engineering
Issue number5-6
Publication statusPublished - May 2005

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology


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