Following mitochondria dynamism: Confocal analysis of the organelle morphology

Francesca R. Mariotti, Mauro Corrado, Silvia Campello

Research output: Contribution to journalArticle

Abstract

Mitochondria are highly dynamic organelles, whose morphology can vary from an elongated and interconnected network to fragmented units. In recent years, outstanding discoveries have linked mitochondrial morphology to the regulation of an increasing number of biological processes, such as biosynthetic pathways, oxidative phosphorylation and ATP production, calcium buffering, and cell death. Here we describe two of the main methods used to analyze the mitochondrial length in fixed cells and the mitochondrial fusion rate in live cells. Moreover, we focus one of the protocols on T cells, as an example of non-adherent cells, which present some particularities and difficulties in the analysis of mitochondrial shape. We also discuss the main mouse models carrying a mitochondrial targeted fluorescent protein, an invaluable tool to deeply investigate in vivo mitochondrial morphology.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalMethods in molecular biology (Clifton, N.J.)
Volume1241
DOIs
Publication statusPublished - 2015

Keywords

  • Fluorescence
  • Fusion and fission
  • Microscopy
  • Mitochondria
  • Morphology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

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