Abstract
Mitochondria are highly dynamic organelles, whose morphology can vary from an elongated and interconnected network to fragmented units. In recent years, outstanding discoveries have linked mitochondrial morphology to the regulation of an increasing number of biological processes, such as biosynthetic pathways, oxidative phosphorylation and ATP production, calcium buffering, and cell death. Here we describe two of the main methods used to analyze the mitochondrial length in fixed cells and the mitochondrial fusion rate in live cells. Moreover, we focus one of the protocols on T cells, as an example of non-adherent cells, which present some particularities and difficulties in the analysis of mitochondrial shape. We also discuss the main mouse models carrying a mitochondrial targeted fluorescent protein, an invaluable tool to deeply investigate in vivo mitochondrial morphology.
| Original language | English |
|---|---|
| Pages (from-to) | 153-161 |
| Number of pages | 9 |
| Journal | Methods in molecular biology (Clifton, N.J.) |
| Volume | 1241 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- Fluorescence
- Fusion and fission
- Microscopy
- Mitochondria
- Morphology
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Medicine(all)
Cite this
Following mitochondria dynamism : Confocal analysis of the organelle morphology. / Mariotti, Francesca R.; Corrado, Mauro; Campello, Silvia.
In: Methods in molecular biology (Clifton, N.J.), Vol. 1241, 2015, p. 153-161.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Following mitochondria dynamism
T2 - Confocal analysis of the organelle morphology
AU - Mariotti, Francesca R.
AU - Corrado, Mauro
AU - Campello, Silvia
PY - 2015
Y1 - 2015
N2 - Mitochondria are highly dynamic organelles, whose morphology can vary from an elongated and interconnected network to fragmented units. In recent years, outstanding discoveries have linked mitochondrial morphology to the regulation of an increasing number of biological processes, such as biosynthetic pathways, oxidative phosphorylation and ATP production, calcium buffering, and cell death. Here we describe two of the main methods used to analyze the mitochondrial length in fixed cells and the mitochondrial fusion rate in live cells. Moreover, we focus one of the protocols on T cells, as an example of non-adherent cells, which present some particularities and difficulties in the analysis of mitochondrial shape. We also discuss the main mouse models carrying a mitochondrial targeted fluorescent protein, an invaluable tool to deeply investigate in vivo mitochondrial morphology.
AB - Mitochondria are highly dynamic organelles, whose morphology can vary from an elongated and interconnected network to fragmented units. In recent years, outstanding discoveries have linked mitochondrial morphology to the regulation of an increasing number of biological processes, such as biosynthetic pathways, oxidative phosphorylation and ATP production, calcium buffering, and cell death. Here we describe two of the main methods used to analyze the mitochondrial length in fixed cells and the mitochondrial fusion rate in live cells. Moreover, we focus one of the protocols on T cells, as an example of non-adherent cells, which present some particularities and difficulties in the analysis of mitochondrial shape. We also discuss the main mouse models carrying a mitochondrial targeted fluorescent protein, an invaluable tool to deeply investigate in vivo mitochondrial morphology.
KW - Fluorescence
KW - Fusion and fission
KW - Microscopy
KW - Mitochondria
KW - Morphology
UR - http://www.scopus.com/inward/record.url?scp=84921809885&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84921809885&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-1875-1_13
DO - 10.1007/978-1-4939-1875-1_13
M3 - Article
VL - 1241
SP - 153
EP - 161
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -