Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation

Dario Acampora, Sylvie Mazan, Yvan Lallemand, Virginia Avantaggiato, Martine Maury, Antonio Simeone, Philippe Brûlet

Research output: Contribution to journalArticle

540 Citations (Scopus)

Abstract

We have replaced part of the mouse homeogene Otx2 coding region with the E. coli lacZ coding sequence, thus creating a null allele of Otx2. By 9.5 dpc, homozygous mutant embryos are characterized by the absence of forebrain and midbrain regions. From the early to midstreak stages, endomesodermal cells expressing lacZ fail to be properly localized anteriorly. In the ectodermal layer, lacZ transcription is progressively extinguished, being barely detectable by the late streak stage. These data suggest that Otx2 expression in endomesoderm and ectoderm is required for anterior neuroectoderm specification. In gastrulating heterozygous embryos, a post-transcriptional repression acts on lacZ transcripts in the ectoderm, but not in the external layer, suggesting that different post-transcriptional mechanisms control Otx2 expression in both layers.

Original languageEnglish
Pages (from-to)3279-3290
Number of pages12
JournalDevelopment
Volume121
Issue number10
Publication statusPublished - Oct 1995

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Neural Plate
Gastrulation
Ectoderm
Prosencephalon
Mesencephalon
Embryonic Structures
Alleles
Escherichia coli

Keywords

  • Anterior neuroectoderm
  • Mouse gastrulation
  • Neural induction
  • Node
  • Otx2

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Acampora, D., Mazan, S., Lallemand, Y., Avantaggiato, V., Maury, M., Simeone, A., & Brûlet, P. (1995). Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. Development, 121(10), 3279-3290.

Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. / Acampora, Dario; Mazan, Sylvie; Lallemand, Yvan; Avantaggiato, Virginia; Maury, Martine; Simeone, Antonio; Brûlet, Philippe.

In: Development, Vol. 121, No. 10, 10.1995, p. 3279-3290.

Research output: Contribution to journalArticle

Acampora, D, Mazan, S, Lallemand, Y, Avantaggiato, V, Maury, M, Simeone, A & Brûlet, P 1995, 'Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation', Development, vol. 121, no. 10, pp. 3279-3290.
Acampora D, Mazan S, Lallemand Y, Avantaggiato V, Maury M, Simeone A et al. Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. Development. 1995 Oct;121(10):3279-3290.
Acampora, Dario ; Mazan, Sylvie ; Lallemand, Yvan ; Avantaggiato, Virginia ; Maury, Martine ; Simeone, Antonio ; Brûlet, Philippe. / Forebrain and midbrain regions are deleted in Otx2-/- mutants due to a defective anterior neuroectoderm specification during gastrulation. In: Development. 1995 ; Vol. 121, No. 10. pp. 3279-3290.
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