Forebrain deletion of αGDI in adult mice worsens the pre-synaptic deficit at cortico-lateral amygdala synaptic connections

Veronica Bianchi, Frédéric Gambino, Luca Muzio, Daniela Toniolo, Yann Humeau, Patrizia D'Adamo

Research output: Contribution to journalArticle

Abstract

The GDI1 gene encodes αGDI, which retrieves inactive GDP-bound RAB from membranes to form a cytosolic pool awaiting vesicular release. Mutations in GDI1 are responsible for X-linked Intellectual Disability. Characterization of the Gdi1-null mice has revealed alterations in the total number and distribution of hippocampal and cortical synaptic vesicles, hippocampal short-term synaptic plasticity and specific short-term memory deficits in adult mice, which are possibly caused by alterations of different synaptic vesicle recycling pathways controlled by several RAB GTPases. However, interpretation of these studies is complicated by the complete ablation of Gdi1 in all cells in the brain throughout development. In this study, we generated conditionally gene-targeted mice in which the knockout of Gdi1 is restricted to the forebrain, hippocampus, cortex and amygdala and occurs only during postnatal development. Adult mutant mice reproduce the short-term memory deficit previously reported in Gdi1-null mice. Surprisingly, the delayed ablation of Gdi1 worsens the pre-synaptic phenotype at cortico-amygdala synaptic connections compared to Gdi1-null mice. These results suggest a pivotal role of αGDI via specific RAB GTPases acting specifically in forebrain regions at the pre-synaptic sites involved in memory formation.

Original languageEnglish
Article numbere29763
JournalPLoS One
Volume7
Issue number1
DOIs
Publication statusPublished - Jan 23 2012

Fingerprint

Guanine Nucleotide Dissociation Inhibitors
amygdala
Prosencephalon
Amygdala
GTP Phosphohydrolases
Ablation
brain
Data storage equipment
mice
Genes
Synaptic Vesicles
Memory Disorders
guanosinetriphosphatase
Short-Term Memory
Plasticity
Recycling
Brain
Membranes
Neuronal Plasticity
postnatal development

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Forebrain deletion of αGDI in adult mice worsens the pre-synaptic deficit at cortico-lateral amygdala synaptic connections. / Bianchi, Veronica; Gambino, Frédéric; Muzio, Luca; Toniolo, Daniela; Humeau, Yann; D'Adamo, Patrizia.

In: PLoS One, Vol. 7, No. 1, e29763, 23.01.2012.

Research output: Contribution to journalArticle

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