Foregut separation and tracheo-oesophageal malformations

The role of tracheal outgrowth, dorso-ventral patterning and programmed cell death

Adonis S. Ioannides, Valentina Massa, Elisabetta Ferraro, Francesco Cecconi, Lewis Spitz, Deborah J. Henderson, Andrew J. Copp

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Foregut division-the separation of dorsal (oesophageal) from ventral (tracheal) foregut components-is a crucial event in gastro-respiratory development, and frequently disturbed in clinical birth defects. Here, we examined three outstanding questions of foregut morphogenesis. The origin of the trachea is suggested to result either from respiratory outgrowth or progressive septation of the foregut tube. We found normal foregut lengthening despite failure of tracheo-oesophageal separation in Adriamycin-treated embryos, whereas active septation was observed only in normal foregut morphogenesis, indicating a primary role for septation. Dorso-ventral patterning of Nkx2.1 (ventral) and Sox2 (dorsal) expression is proposed to be critical for tracheo-oesophageal separation. However, normal dorso-ventral patterning of Nkx2.1 and Sox2 expression occurred in Adriamycin-treated embryos with defective foregut separation. In contrast, Shh expression shifts dynamically, ventral-to-dorsal, solely during normal morphogenesis, particularly implicating Shh in foregut morphogenesis. Dying cells localise to the fusing foregut epithelial ridges, with disturbance of this apoptotic pattern in Adriamycin, Shh and Nkx2.1 models. Strikingly, however, genetic suppression of apoptosis in the Apaf1 mutant did not prevent foregut separation, indicating that apoptosis is not required for tracheo-oesophageal morphogenesis. Epithelial remodelling during septation may cause loss of cell-cell or cell-matrix interactions, resulting in apoptosis (anoikis) as a secondary consequence.

Original languageEnglish
Pages (from-to)351-362
Number of pages12
JournalDevelopmental Biology
Volume337
Issue number2
DOIs
Publication statusPublished - Jan 15 2010

Fingerprint

Morphogenesis
Cell Death
Doxorubicin
Apoptosis
Embryonic Structures
Anoikis
Genetic Suppression
Trachea
Cell Communication

Keywords

  • Adriamycin
  • Anoikis
  • Apoptosis
  • Cell proliferation
  • Embryo
  • Malformations
  • Mouse
  • Oesophagus
  • Trachea
  • Tracheo-oesophageal defects

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Foregut separation and tracheo-oesophageal malformations : The role of tracheal outgrowth, dorso-ventral patterning and programmed cell death. / Ioannides, Adonis S.; Massa, Valentina; Ferraro, Elisabetta; Cecconi, Francesco; Spitz, Lewis; Henderson, Deborah J.; Copp, Andrew J.

In: Developmental Biology, Vol. 337, No. 2, 15.01.2010, p. 351-362.

Research output: Contribution to journalArticle

Ioannides, Adonis S. ; Massa, Valentina ; Ferraro, Elisabetta ; Cecconi, Francesco ; Spitz, Lewis ; Henderson, Deborah J. ; Copp, Andrew J. / Foregut separation and tracheo-oesophageal malformations : The role of tracheal outgrowth, dorso-ventral patterning and programmed cell death. In: Developmental Biology. 2010 ; Vol. 337, No. 2. pp. 351-362.
@article{c0c054c374a44963866d08eee7c52d62,
title = "Foregut separation and tracheo-oesophageal malformations: The role of tracheal outgrowth, dorso-ventral patterning and programmed cell death",
abstract = "Foregut division-the separation of dorsal (oesophageal) from ventral (tracheal) foregut components-is a crucial event in gastro-respiratory development, and frequently disturbed in clinical birth defects. Here, we examined three outstanding questions of foregut morphogenesis. The origin of the trachea is suggested to result either from respiratory outgrowth or progressive septation of the foregut tube. We found normal foregut lengthening despite failure of tracheo-oesophageal separation in Adriamycin-treated embryos, whereas active septation was observed only in normal foregut morphogenesis, indicating a primary role for septation. Dorso-ventral patterning of Nkx2.1 (ventral) and Sox2 (dorsal) expression is proposed to be critical for tracheo-oesophageal separation. However, normal dorso-ventral patterning of Nkx2.1 and Sox2 expression occurred in Adriamycin-treated embryos with defective foregut separation. In contrast, Shh expression shifts dynamically, ventral-to-dorsal, solely during normal morphogenesis, particularly implicating Shh in foregut morphogenesis. Dying cells localise to the fusing foregut epithelial ridges, with disturbance of this apoptotic pattern in Adriamycin, Shh and Nkx2.1 models. Strikingly, however, genetic suppression of apoptosis in the Apaf1 mutant did not prevent foregut separation, indicating that apoptosis is not required for tracheo-oesophageal morphogenesis. Epithelial remodelling during septation may cause loss of cell-cell or cell-matrix interactions, resulting in apoptosis (anoikis) as a secondary consequence.",
keywords = "Adriamycin, Anoikis, Apoptosis, Cell proliferation, Embryo, Malformations, Mouse, Oesophagus, Trachea, Tracheo-oesophageal defects",
author = "Ioannides, {Adonis S.} and Valentina Massa and Elisabetta Ferraro and Francesco Cecconi and Lewis Spitz and Henderson, {Deborah J.} and Copp, {Andrew J.}",
year = "2010",
month = "1",
day = "15",
doi = "10.1016/j.ydbio.2009.11.005",
language = "English",
volume = "337",
pages = "351--362",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Foregut separation and tracheo-oesophageal malformations

T2 - The role of tracheal outgrowth, dorso-ventral patterning and programmed cell death

AU - Ioannides, Adonis S.

AU - Massa, Valentina

AU - Ferraro, Elisabetta

AU - Cecconi, Francesco

AU - Spitz, Lewis

AU - Henderson, Deborah J.

AU - Copp, Andrew J.

PY - 2010/1/15

Y1 - 2010/1/15

N2 - Foregut division-the separation of dorsal (oesophageal) from ventral (tracheal) foregut components-is a crucial event in gastro-respiratory development, and frequently disturbed in clinical birth defects. Here, we examined three outstanding questions of foregut morphogenesis. The origin of the trachea is suggested to result either from respiratory outgrowth or progressive septation of the foregut tube. We found normal foregut lengthening despite failure of tracheo-oesophageal separation in Adriamycin-treated embryos, whereas active septation was observed only in normal foregut morphogenesis, indicating a primary role for septation. Dorso-ventral patterning of Nkx2.1 (ventral) and Sox2 (dorsal) expression is proposed to be critical for tracheo-oesophageal separation. However, normal dorso-ventral patterning of Nkx2.1 and Sox2 expression occurred in Adriamycin-treated embryos with defective foregut separation. In contrast, Shh expression shifts dynamically, ventral-to-dorsal, solely during normal morphogenesis, particularly implicating Shh in foregut morphogenesis. Dying cells localise to the fusing foregut epithelial ridges, with disturbance of this apoptotic pattern in Adriamycin, Shh and Nkx2.1 models. Strikingly, however, genetic suppression of apoptosis in the Apaf1 mutant did not prevent foregut separation, indicating that apoptosis is not required for tracheo-oesophageal morphogenesis. Epithelial remodelling during septation may cause loss of cell-cell or cell-matrix interactions, resulting in apoptosis (anoikis) as a secondary consequence.

AB - Foregut division-the separation of dorsal (oesophageal) from ventral (tracheal) foregut components-is a crucial event in gastro-respiratory development, and frequently disturbed in clinical birth defects. Here, we examined three outstanding questions of foregut morphogenesis. The origin of the trachea is suggested to result either from respiratory outgrowth or progressive septation of the foregut tube. We found normal foregut lengthening despite failure of tracheo-oesophageal separation in Adriamycin-treated embryos, whereas active septation was observed only in normal foregut morphogenesis, indicating a primary role for septation. Dorso-ventral patterning of Nkx2.1 (ventral) and Sox2 (dorsal) expression is proposed to be critical for tracheo-oesophageal separation. However, normal dorso-ventral patterning of Nkx2.1 and Sox2 expression occurred in Adriamycin-treated embryos with defective foregut separation. In contrast, Shh expression shifts dynamically, ventral-to-dorsal, solely during normal morphogenesis, particularly implicating Shh in foregut morphogenesis. Dying cells localise to the fusing foregut epithelial ridges, with disturbance of this apoptotic pattern in Adriamycin, Shh and Nkx2.1 models. Strikingly, however, genetic suppression of apoptosis in the Apaf1 mutant did not prevent foregut separation, indicating that apoptosis is not required for tracheo-oesophageal morphogenesis. Epithelial remodelling during septation may cause loss of cell-cell or cell-matrix interactions, resulting in apoptosis (anoikis) as a secondary consequence.

KW - Adriamycin

KW - Anoikis

KW - Apoptosis

KW - Cell proliferation

KW - Embryo

KW - Malformations

KW - Mouse

KW - Oesophagus

KW - Trachea

KW - Tracheo-oesophageal defects

UR - http://www.scopus.com/inward/record.url?scp=72649104363&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72649104363&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2009.11.005

DO - 10.1016/j.ydbio.2009.11.005

M3 - Article

VL - 337

SP - 351

EP - 362

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -