Formate binding to ferric wild type and mutant myoglobins thermodynamic and X-ray crystallographic study

Edlira Leci, Andrea Brancaccio, Francesca Cutruzzolà, Carlo Travaglini Allocatelli, Cataldo Tarricone, Martino Bolognesi, Alessandro Desideri, Paolo Ascenzi

Research output: Contribution to journalArticle

Abstract

The X-ray crystal structure of the formate derivative of ferric loggerhead sea turtle (Caretta caretta) Mb has been determined at 2.0 Å resolution (R = 0.164) by difference Fourier techniques. Formate, sitting in the central part of the heme distal site, is coordinated to the heme iron as unidentate ligand, through the O1 oxygen atom, and is hydrogen bonded to the distal His64(E7) NE2 atom through O2. Thermodynamics for formate binding to ferric loggerhead sea turtle Mb, sperm whale Mb, Aplysia limacina Mb, as well as to the VR and VRS mutants of sperm whale Mb were obtained between pH 4.5 and 8.5, at 20.0°C. These results, representing the first structure of a ferric hemoprotein:formate complex solved by X-ray crystallography, outline the role of amino acid residues at positions E7, F8 and E10 in modulating ligand binding properties of oxygen carrying proteins.

Original languageEnglish
Pages (from-to)227-229
Number of pages3
JournalFEBS Letters
Volume357
Issue number3
DOIs
Publication statusPublished - Jan 9 1995

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Keywords

  • Aplysia limacina myoglobin
  • Formate binding
  • Loggerhead sea turtle (Caretta caretta) myoglobin
  • Sperm whale (Physeter catodon) myoglobin
  • Thermodynamics
  • Wild type and mutant myoglobin
  • X-ray crystal structure (of the ferric loggerhead sea turtle myoglobin:formate complex)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Leci, E., Brancaccio, A., Cutruzzolà, F., Allocatelli, C. T., Tarricone, C., Bolognesi, M., Desideri, A., & Ascenzi, P. (1995). Formate binding to ferric wild type and mutant myoglobins thermodynamic and X-ray crystallographic study. FEBS Letters, 357(3), 227-229. https://doi.org/10.1016/0014-5793(94)01324-T