Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells

Anita Muraglia, Alessandro Corsi, Mara Riminucci, Maddalena Mastrogiacomo, Ranieri Cancedda, Paolo Bianco, Rodolfo Quarto

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Bone-marrow stromal cells can differentiate into multiple mesenchymal lineages including cartilage and bone. When these cells are seeded in high-density 'pellet culture', they undergo chondrogenesis and form a tissue that is morphologically and biochemically defined as cartilage. Here, we show that dual chondro-osteogenic differentiation can be obtained in the same micromass culture of human bone-marrow stromal cells. Human bone-marrow stromal cells were pellet cultured for 4 weeks in chondro-inductive medium. Cartilage 'beads' resulting from the micromass culture were then subcultured for further 1-3 weeks in osteo-inductive medium. This resulted in the formation of a distinct mineralized bony collar around hyaline cartilage. During the chondrogenesis phase, type I collagen and bone sialoprotein were produced in the outer portion of the cartilage bead, which, upon subsequent exposure to β-glycerophosphate, mineralized and accumulated extracellular bone sialoprotein and osteocalcin. Our modification of the pellet culture system results in the formation of a chondro-osseous 'organoid' structurally reminiscent of pre-invasion endochondral rudiments, in which a bony collar forms around hyaline cartilage. The transition from a cell culture to an organ culture dimension featured by our system provides a suitable model for the dissection of molecular determinants of endochondral bone formation, which unfolds in a precisely defined spatial and temporal frame.

Original languageEnglish
Pages (from-to)2949-2955
Number of pages7
JournalJournal of Cell Science
Volume116
Issue number14
DOIs
Publication statusPublished - Jul 15 2003

Fingerprint

Mesenchymal Stromal Cells
Cartilage
Hyaline Cartilage
Chondrogenesis
Organoids
Integrin-Binding Sialoprotein
Glycerophosphates
Osteopontin
Molecular Models
Organ Culture Techniques
Osteocalcin
Collagen Type I
Osteogenesis
Dissection
Cell Culture Techniques
Bone and Bones

Keywords

  • Bone-marrow stromal cells
  • Chondrogenesis
  • Condensation
  • Osteogenesis
  • Osteoprogenitors

ASJC Scopus subject areas

  • Cell Biology

Cite this

Muraglia, A., Corsi, A., Riminucci, M., Mastrogiacomo, M., Cancedda, R., Bianco, P., & Quarto, R. (2003). Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells. Journal of Cell Science, 116(14), 2949-2955. https://doi.org/10.1242/jcs.00527

Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells. / Muraglia, Anita; Corsi, Alessandro; Riminucci, Mara; Mastrogiacomo, Maddalena; Cancedda, Ranieri; Bianco, Paolo; Quarto, Rodolfo.

In: Journal of Cell Science, Vol. 116, No. 14, 15.07.2003, p. 2949-2955.

Research output: Contribution to journalArticle

Muraglia, A, Corsi, A, Riminucci, M, Mastrogiacomo, M, Cancedda, R, Bianco, P & Quarto, R 2003, 'Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells', Journal of Cell Science, vol. 116, no. 14, pp. 2949-2955. https://doi.org/10.1242/jcs.00527
Muraglia A, Corsi A, Riminucci M, Mastrogiacomo M, Cancedda R, Bianco P et al. Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells. Journal of Cell Science. 2003 Jul 15;116(14):2949-2955. https://doi.org/10.1242/jcs.00527
Muraglia, Anita ; Corsi, Alessandro ; Riminucci, Mara ; Mastrogiacomo, Maddalena ; Cancedda, Ranieri ; Bianco, Paolo ; Quarto, Rodolfo. / Formation of a chondro-osseous rudiment in micromass cultures of human bone-marrow stromal cells. In: Journal of Cell Science. 2003 ; Vol. 116, No. 14. pp. 2949-2955.
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