Formation of a cytosolic ganglioside-protein complex following administration of photoreactive ganglioside GM1 to human fibroblasts in culture

Vanna Chigorno; Manuela Valsecchi; Domenico Acquotti; Sandro Sonnino; Guido Tettamanti

doi:10.1016/0014-5793(90)81406-E

Formation of a cytosolic ganglioside-protein complex following administration of photoreactive ganglioside GM1 to human fibroblasts in culture

Vanna Chigorno, Manuela Valsecchi, Domenico Acquotti, Sandro Sonnino, Guido Tettamanti

IRCCS Policlinico San Donato

Research output: Contribution to journal › Article › peer-review

Abstract

A GM1 ganglioside derivative bearing a photoreactive nitrophenyl azide group and tritium labeled at the acetyl group of N-acetylneuraminic acid, has been administered to cultured human fibroblasts. With photolabeling experiments we found that a portion of the ganglioside in the cell cytosol was associated with a soluble protein of about 30 kDa.

Original language	English
Pages (from-to)	329-331
Number of pages	3
Journal	FEBS Letters
Volume	263
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(90)81406-E
Publication status	Published - Apr 24 1990

Keywords

Cytosolic complex
Ganglioside-protein interaction
Photoreactive GM1

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Cell Biology
Genetics
Structural Biology

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10.1016/0014-5793(90)81406-E

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abstract = "A GM1 ganglioside derivative bearing a photoreactive nitrophenyl azide group and tritium labeled at the acetyl group of N-acetylneuraminic acid, has been administered to cultured human fibroblasts. With photolabeling experiments we found that a portion of the ganglioside in the cell cytosol was associated with a soluble protein of about 30 kDa.",

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AU - Acquotti, Domenico

AU - Sonnino, Sandro

AU - Tettamanti, Guido

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