Formation of a cytosolic ganglioside-protein complex following administration of photoreactive ganglioside GM1 to human fibroblasts in culture

Vanna Chigorno, Manuela Valsecchi, Domenico Acquotti, Sandro Sonnino, Guido Tettamanti

Research output: Contribution to journalArticlepeer-review

Abstract

A GM1 ganglioside derivative bearing a photoreactive nitrophenyl azide group and tritium labeled at the acetyl group of N-acetylneuraminic acid, has been administered to cultured human fibroblasts. With photolabeling experiments we found that a portion of the ganglioside in the cell cytosol was associated with a soluble protein of about 30 kDa.

Original languageEnglish
Pages (from-to)329-331
Number of pages3
JournalFEBS Letters
Volume263
Issue number2
DOIs
Publication statusPublished - Apr 24 1990

Keywords

  • Cytosolic complex
  • Ganglioside-protein interaction
  • Photoreactive GM1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

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