Formation of self-assembled triple-layered rotavirus-like particles (tlRLPs) by constitutive co-expression of VP2, VP6, and VP7 in stably transfected high-five insect cell lines

Zabihollah Shoja, Maria Tagliamonte, Somayeh Jalilvand, Yaghoub Mollaei-Kandelous, Angelo De stradis, Maria Lina Tornesello, Franco M. Buonaguro, Luigi Buonaguro

Research output: Contribution to journalArticle

Abstract

In this study, stable high-five insect cell line constitutively expressing rotavirus (RV) VP2 was co-transfected with VP6 and VP7-recombinant plasmids. The presence of RV proteins in stably transfected high-five cells was verified by molecular and protein analyses. To yield self-assembled triple-layered RV-like particles (tlRLPs), a stable insect high-five cell line was generated to produce RV VP6 and VP7 besides VP2. Self-assembled tlRLPs were observed by transmission electron microscopy (TEM), and enzyme-linked immunosorbent assay (ELISA) was used to assess their antigenicity in vivo. The results suggest that the stable transfected high-five cells are able to generate tlRLPs with the efficient antigenicity.

Original languageEnglish
Pages (from-to)102-111
Number of pages10
JournalJournal of Medical Virology
Volume87
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Keywords

  • Antigenicity
  • High-five insect cells
  • Rotavirus VP2, VP6, and VP7
  • TlRLP

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Medicine(all)

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