Formation of the 3' end on U snRNAs requires at least three sequence elements.

G. Ciliberto, N. Dathan, R. Frank, L. Philipson, I. W. Mattaj

Research output: Contribution to journalArticle

Abstract

The structural requirements for 3' end formation on the Xenopus laevis U1B snRNA gene have been studied. Three sequence elements are shown to be required. The first is a conserved sequence element found immediately 3' of all vertebrate U snRNA genes studied so far. The second is a gene internal sequence potentially capable of forming a stem-loop structure close to the 3' end of the RNA. The third element lies upstream of these, and may be part of the gene promoter. Experiments designed to investigate the mechanism of 3' end formation on primary U1B snRNA transcripts failed to find evidence for a processing event.

Original languageEnglish
Pages (from-to)2931-2937
Number of pages7
JournalEMBO Journal
Volume5
Issue number11
Publication statusPublished - Nov 1986

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ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Ciliberto, G., Dathan, N., Frank, R., Philipson, L., & Mattaj, I. W. (1986). Formation of the 3' end on U snRNAs requires at least three sequence elements. EMBO Journal, 5(11), 2931-2937.