Formation of three-dimensional protein-lipid aggregates in monolayer films induced by surfactant protein B

Silke Krol, Michaela Ross, Manfred Sieber, Stephanie Kunneke, Hans Joachim Galla, Andreas Janshoff

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

This study focuses on the structural organization of surfactant protein B (SP-B) containing lipid monolayers. The artificial system is composed of the saturated phospholipids dipalmitoylphosphatidylcholine (DPPC) and dipalmitoylphosphatidylglycerol (DPPG) in a molar ratio of 4:1 with 0.2 mol% SP-B. The different 'squeeze-out' structures of SP-B were visualized by scanning probe microscopy and compared with structures formed by SP-C. Particularly, the morphology and material properties of mixed monolayers containing 0.2 mol% SP-B in a wide pressure range of 10 to 54 mN/m were investigated revealing that filamentous domain boundaries occur at intermediate surface pressure (15-30 mN/m), while disc-like protrusions prevail at elevated pressure (50-54 mN/m). In contrast, SP-C containing lipid monolayers exhibit large flat protrusions composed of stacked bilayers in the plateau region (app. 52 mN/m) of the pressure-area isotherm. By using different scanning probe techniques (lateral force microscopy, force modulation, phase imaging) it was shown that SP-B is dissolved in the liquid expanded rather than in the liquid condensed phase of the monolayer. Although artificial, the investigation of this system contributes to further understanding of the function of lung surfactant in the alveolus.

Original languageEnglish
Pages (from-to)904-918
Number of pages15
JournalBiophysical Journal
Volume79
Issue number2
Publication statusPublished - 2000

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Surface-Active Agents
Lipids
Pressure
Scanning Probe Microscopy
1,2-Dipalmitoylphosphatidylcholine
Atomic Force Microscopy
Phospholipids
IgA receptor
Protein Aggregates
Lung

ASJC Scopus subject areas

  • Biophysics

Cite this

Krol, S., Ross, M., Sieber, M., Kunneke, S., Galla, H. J., & Janshoff, A. (2000). Formation of three-dimensional protein-lipid aggregates in monolayer films induced by surfactant protein B. Biophysical Journal, 79(2), 904-918.

Formation of three-dimensional protein-lipid aggregates in monolayer films induced by surfactant protein B. / Krol, Silke; Ross, Michaela; Sieber, Manfred; Kunneke, Stephanie; Galla, Hans Joachim; Janshoff, Andreas.

In: Biophysical Journal, Vol. 79, No. 2, 2000, p. 904-918.

Research output: Contribution to journalArticle

Krol, S, Ross, M, Sieber, M, Kunneke, S, Galla, HJ & Janshoff, A 2000, 'Formation of three-dimensional protein-lipid aggregates in monolayer films induced by surfactant protein B', Biophysical Journal, vol. 79, no. 2, pp. 904-918.
Krol, Silke ; Ross, Michaela ; Sieber, Manfred ; Kunneke, Stephanie ; Galla, Hans Joachim ; Janshoff, Andreas. / Formation of three-dimensional protein-lipid aggregates in monolayer films induced by surfactant protein B. In: Biophysical Journal. 2000 ; Vol. 79, No. 2. pp. 904-918.
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