Formation of tritium-labeled polysialylated gangliosides in the cytosol of rat cerebellar granule cells in culture following administration of [3H]GM1 ganglioside

Vanna Chigorno, Manuela Valsecchi, Sandro Sonnino, Guido Tettamanti

Research output: Contribution to journalArticlepeer-review

Abstract

GM1 ganglioside tritium-labeled at C-3 of sphingosine has been administered to rat cerebellar granule cells. Tritiated polysialylated gangliosides were observed in the cytosol of the cells, where they resulted in a higher amount after a short period of chase. This, together with the data showing an increase of the tritiated polysialylated gangliosides in the total particulate fraction in parallel to the prolonging of the chase period, suggests that cytosolic gangliosides could be a way of transporting neosynthesized gangliosides from the Golgi apparatus to the plasma membranes.

Original languageEnglish
Pages (from-to)164-166
Number of pages3
JournalFEBS Letters
Volume277
Issue number1-2
DOIs
Publication statusPublished - Dec 17 1990

Keywords

  • Biosynthesis
  • Cytosol
  • Ganglioside
  • Transport

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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