FOSL1 as a candidate target gene for 11 q 12 rearrangements in desmoplastic fibroblastoma

Gemma MacChia, Domenico Trombetta, Emely Möller, Fredrik Mertens, Clelia T. Storlazzi, Maria Debiec-Rychter, Raf Sciot, Karolin H. Nord

Research output: Contribution to journalArticle

Abstract

Desmoplastic fibroblastoma (DF) is a benign fibroblastic/myofibroblastic tumor. Cytogenetic analyses have revealed consistent rearrangement of chromosome band 11q12, strongly suggesting that this region harbors a gene of pathogenetic importance. To identify the target gene of the 11q12 rearrangements, we analyzed six cases diagnosed as DF using chromosome banding, fluorescence in situ hybridization (FISH), single-nucleotide polymorphism array and gene expression approaches. Different structural rearrangements involving 11q12 were found in five of the six cases. Metaphase FISH analyses in two of them mapped the 11q12 breakpoints to an ≈20-kb region, harboring FOSL1. Global gene expression profiling followed by quantitative real-time PCR showed that FOSL1 was expressed at higher levels in DF with 11q12 rearrangements than in desmoid-type fibromatoses. Furthermore, FOSL1 was not upregulated in the single case of DF that did not show cytogenetic involvement of 11q12; instead this tumor was found to display a hemizygous loss on 5q, including the APC (adenomatous polyposis coli) locus, raising the possibility that it actually was a misdiagnosed Gardner fibroma. 5′RACE-PCR in two 11q12-positive DF did not identify any fusion transcripts. Thus, in agreement with the finding at chromosome banding analysis that varying translocation partners are involved in the 11q12 rearrangement, the molecular data suggest that the functional outcome of the 11q12 rearrangements is deregulated expression of FOSL1.

Original languageEnglish
Pages (from-to)735-743
Number of pages9
JournalLaboratory Investigation
Volume92
Issue number5
DOIs
Publication statusPublished - May 2012

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Keywords

  • 11q12
  • APC
  • CTNNB1
  • Desmoid tumor
  • Desmoplastic fibroblastoma
  • FOSL1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Cell Biology
  • Molecular Biology

Cite this

MacChia, G., Trombetta, D., Möller, E., Mertens, F., Storlazzi, C. T., Debiec-Rychter, M., Sciot, R., & Nord, K. H. (2012). FOSL1 as a candidate target gene for 11 q 12 rearrangements in desmoplastic fibroblastoma. Laboratory Investigation, 92(5), 735-743. https://doi.org/10.1038/labinvest.2012.46