Four phases of checks for exclusion of umbilical cord blood donors

Gennaro Volpe, Michele Santoditocco, Lazzaro Di Mauro, Giuseppe Miscio, Filippo Maria Boscia, Brunella Muto, Nicola Volpe

Research output: Contribution to journalArticlepeer-review

Abstract

Aim. The aim of this study was to analyse umbilical cord blood (UCB) collection over 1 year between October 2008 and September 2009, seeking ways to improve the number of suitable banked UCB units. Four phases of the process were investigated, from the consent form to the banking procedure, paying attention to the discarded UCB units. Material and methods. We recruited couples at 35 weeks of gestation and took an accurate history, focusing on genetic, immunological and infectious diseases. We collected UCB from pregnant women who delivered vaginally or by Caesarean section between the 37-41+6 weeks of gestation. Some units were discarded on the basis of the patients' history, obstetric events or biological criteria. In utero collection was the preferred method of collection. Results. During the study period, between October 2008 and September 2009, there were 1,477 deliveries in our unit. The number of couples interested in UCB donation was 595 (40.2%-595/1,477). We collected 393 UBC units. We excluded 122 patients at the phase of the history taking, counselling and informed consent (first phase check). Of the 393 units collected, 162 (41.3%) were banked whereas 231 (58.7%) were discarded because they did not fulfil biological criteria (third phase check). The volume of UCB units collected after Caesarean section was greater than the volume of units collected after vaginal delivery (95.4 mL versus 85.0 mL, respectively; p 6 cells versus 874×106 cells, respectively; p=0.037). None of the banked UCB units was discarded at the clinical check 6 months after delivery (fourth phase check). Conclusions. Our study shows that strict observance of each of the checks and the collection strategy is important to guarantee the safety of the UCB units and to maximise the cost-benefit ratio. After the appropriate checks we banked UCB units from only 27.2% (162/595) of the couples who gave consent to the procedure and from only 11% (162/1,477) of all the deliveries in the 12 month study period, as 59.8% of couples were not properly informed about UCB donation.

Original languageEnglish
Pages (from-to)286-291
Number of pages6
JournalBlood Transfusion
Volume9
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • Cord blood
  • Obstetric factors
  • Stem cells

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

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