Four-week ingestion of blood orange juice results in measurable anthocyanin urinary levels but does not affect cellular markers related to cardiovascular risk

A randomized cross-over study in healthy volunteers

Lucia Giordano, Walter Coletta, Chiara Tamburrelli, Marco D'Imperio, Marilena Crescente, Cristian Silvestri, Paolo Rapisarda, Giuseppe Reforgiato Recupero, Amalia De Curtis, Licia Iacoviello, Giovanni De Gaetano, Domenico Rotilio, Chiara Cerletti, Maria Benedetta Donati

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose: Blood orange juice (OJ) is an important source of anthocyanins (ACN). The latter molecules are endowed with antioxidant activity and might thus modulate different cell function. Our aim was to investigate ACN absorption following a 1-month daily supplementation of blood OJ and their potential effects on cell markers of platelet and leukocyte activation and interaction. Methods: Eighteen healthy subjects (10 men and 8 women) were supplemented for 4 weeks with 1 L/day of either blood OJ or blond OJ (that contains no ACN), following a cross-over design. Blood samples were obtained from fasting participants both at baseline and after each week of treatment to measure plasma ACN concentration. At the same time-intervals, 24-h urinary excretion of these molecules was also measured. At the beginning and the end of each 4-week intervention period, platelet and leukocyte markers and mixed cell conjugates were assessed both in basal condition and upon in vitro collagen/ADP activation. Results After 1 week supplementation with blood OJ, 24-h urinary excretion of ACN reached average levels of 11.47 ± 5.63 nmol that significantly differed from baseline and remained substantially unchanged until the end of treatment. No plasma accumulation of ACN following blood OJ supplementation was observed. Cellular markers were not significantly affected by either OJ after 4-week supplementation. Conclusions: Following supplementation of healthy volunteers with 1 L/day of blood OJ for 4 weeks, the ACN plasma levels reached were insufficient to significantly modify cell markers of platelet and leukocyte activation and interaction.

Original languageEnglish
Pages (from-to)541-548
Number of pages8
JournalEuropean Journal of Nutrition
Volume51
Issue number5
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Anthocyanins
Cross-Over Studies
Healthy Volunteers
Eating
Leukocytes
Platelet Activation
Adenosine Diphosphate
Fasting
Collagen
Blood Platelets
Antioxidants
Therapeutics

Keywords

  • Anthocyanins
  • Bioavailability
  • Blood orange juice
  • Leukocytes
  • Platelets

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Four-week ingestion of blood orange juice results in measurable anthocyanin urinary levels but does not affect cellular markers related to cardiovascular risk : A randomized cross-over study in healthy volunteers. / Giordano, Lucia; Coletta, Walter; Tamburrelli, Chiara; D'Imperio, Marco; Crescente, Marilena; Silvestri, Cristian; Rapisarda, Paolo; Reforgiato Recupero, Giuseppe; De Curtis, Amalia; Iacoviello, Licia; De Gaetano, Giovanni; Rotilio, Domenico; Cerletti, Chiara; Donati, Maria Benedetta.

In: European Journal of Nutrition, Vol. 51, No. 5, 08.2012, p. 541-548.

Research output: Contribution to journalArticle

Giordano, Lucia ; Coletta, Walter ; Tamburrelli, Chiara ; D'Imperio, Marco ; Crescente, Marilena ; Silvestri, Cristian ; Rapisarda, Paolo ; Reforgiato Recupero, Giuseppe ; De Curtis, Amalia ; Iacoviello, Licia ; De Gaetano, Giovanni ; Rotilio, Domenico ; Cerletti, Chiara ; Donati, Maria Benedetta. / Four-week ingestion of blood orange juice results in measurable anthocyanin urinary levels but does not affect cellular markers related to cardiovascular risk : A randomized cross-over study in healthy volunteers. In: European Journal of Nutrition. 2012 ; Vol. 51, No. 5. pp. 541-548.
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abstract = "Purpose: Blood orange juice (OJ) is an important source of anthocyanins (ACN). The latter molecules are endowed with antioxidant activity and might thus modulate different cell function. Our aim was to investigate ACN absorption following a 1-month daily supplementation of blood OJ and their potential effects on cell markers of platelet and leukocyte activation and interaction. Methods: Eighteen healthy subjects (10 men and 8 women) were supplemented for 4 weeks with 1 L/day of either blood OJ or blond OJ (that contains no ACN), following a cross-over design. Blood samples were obtained from fasting participants both at baseline and after each week of treatment to measure plasma ACN concentration. At the same time-intervals, 24-h urinary excretion of these molecules was also measured. At the beginning and the end of each 4-week intervention period, platelet and leukocyte markers and mixed cell conjugates were assessed both in basal condition and upon in vitro collagen/ADP activation. Results After 1 week supplementation with blood OJ, 24-h urinary excretion of ACN reached average levels of 11.47 ± 5.63 nmol that significantly differed from baseline and remained substantially unchanged until the end of treatment. No plasma accumulation of ACN following blood OJ supplementation was observed. Cellular markers were not significantly affected by either OJ after 4-week supplementation. Conclusions: Following supplementation of healthy volunteers with 1 L/day of blood OJ for 4 weeks, the ACN plasma levels reached were insufficient to significantly modify cell markers of platelet and leukocyte activation and interaction.",
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T1 - Four-week ingestion of blood orange juice results in measurable anthocyanin urinary levels but does not affect cellular markers related to cardiovascular risk

T2 - A randomized cross-over study in healthy volunteers

AU - Giordano, Lucia

AU - Coletta, Walter

AU - Tamburrelli, Chiara

AU - D'Imperio, Marco

AU - Crescente, Marilena

AU - Silvestri, Cristian

AU - Rapisarda, Paolo

AU - Reforgiato Recupero, Giuseppe

AU - De Curtis, Amalia

AU - Iacoviello, Licia

AU - De Gaetano, Giovanni

AU - Rotilio, Domenico

AU - Cerletti, Chiara

AU - Donati, Maria Benedetta

PY - 2012/8

Y1 - 2012/8

N2 - Purpose: Blood orange juice (OJ) is an important source of anthocyanins (ACN). The latter molecules are endowed with antioxidant activity and might thus modulate different cell function. Our aim was to investigate ACN absorption following a 1-month daily supplementation of blood OJ and their potential effects on cell markers of platelet and leukocyte activation and interaction. Methods: Eighteen healthy subjects (10 men and 8 women) were supplemented for 4 weeks with 1 L/day of either blood OJ or blond OJ (that contains no ACN), following a cross-over design. Blood samples were obtained from fasting participants both at baseline and after each week of treatment to measure plasma ACN concentration. At the same time-intervals, 24-h urinary excretion of these molecules was also measured. At the beginning and the end of each 4-week intervention period, platelet and leukocyte markers and mixed cell conjugates were assessed both in basal condition and upon in vitro collagen/ADP activation. Results After 1 week supplementation with blood OJ, 24-h urinary excretion of ACN reached average levels of 11.47 ± 5.63 nmol that significantly differed from baseline and remained substantially unchanged until the end of treatment. No plasma accumulation of ACN following blood OJ supplementation was observed. Cellular markers were not significantly affected by either OJ after 4-week supplementation. Conclusions: Following supplementation of healthy volunteers with 1 L/day of blood OJ for 4 weeks, the ACN plasma levels reached were insufficient to significantly modify cell markers of platelet and leukocyte activation and interaction.

AB - Purpose: Blood orange juice (OJ) is an important source of anthocyanins (ACN). The latter molecules are endowed with antioxidant activity and might thus modulate different cell function. Our aim was to investigate ACN absorption following a 1-month daily supplementation of blood OJ and their potential effects on cell markers of platelet and leukocyte activation and interaction. Methods: Eighteen healthy subjects (10 men and 8 women) were supplemented for 4 weeks with 1 L/day of either blood OJ or blond OJ (that contains no ACN), following a cross-over design. Blood samples were obtained from fasting participants both at baseline and after each week of treatment to measure plasma ACN concentration. At the same time-intervals, 24-h urinary excretion of these molecules was also measured. At the beginning and the end of each 4-week intervention period, platelet and leukocyte markers and mixed cell conjugates were assessed both in basal condition and upon in vitro collagen/ADP activation. Results After 1 week supplementation with blood OJ, 24-h urinary excretion of ACN reached average levels of 11.47 ± 5.63 nmol that significantly differed from baseline and remained substantially unchanged until the end of treatment. No plasma accumulation of ACN following blood OJ supplementation was observed. Cellular markers were not significantly affected by either OJ after 4-week supplementation. Conclusions: Following supplementation of healthy volunteers with 1 L/day of blood OJ for 4 weeks, the ACN plasma levels reached were insufficient to significantly modify cell markers of platelet and leukocyte activation and interaction.

KW - Anthocyanins

KW - Bioavailability

KW - Blood orange juice

KW - Leukocytes

KW - Platelets

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