Fractional incorporation of radionucleotides, a marker of in vitro tumor cell chemosensitivity in colorectal cancer

Fabrizio Franchi; Patrizia Seminara; Diana Giannarelli; Nina Konovalova

doi:10.1159/000226991

Fractional incorporation of radionucleotides, a marker of in vitro tumor cell chemosensitivity in colorectal cancer

Fabrizio Franchi, Patrizia Seminara, Diana Giannarelli, Nina Konovalova

Istituto Regina Elena

Research output: Contribution to journal › Article › peer-review

Abstract

Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four difTerent drugs (5-FU. mitomycin C, 4¢-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of³H-TdR and³H-UdR incorporation into DN A and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.

Original language	English
Pages (from-to)	510-516
Number of pages	7
Journal	Oncology
Volume	48
Issue number	6
DOIs	https://doi.org/10.1159/000226991
Publication status	Published - 1991

Keywords

5-Fluorouracil
Cell kinetics
Chemosensitivity assays
Colorectal cancer
Mitomycin C
Tumor chemotherapy

ASJC Scopus subject areas

Cancer Research
Oncology

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10.1159/000226991

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abstract = "Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four difTerent drugs (5-FU. mitomycin C, 4¢-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of3H-TdR and3H-UdR incorporation into DN A and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.",

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journal = "Oncology",

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T1 - Fractional incorporation of radionucleotides, a marker of in vitro tumor cell chemosensitivity in colorectal cancer

AU - Franchi, Fabrizio

AU - Seminara, Patrizia

AU - Giannarelli, Diana

AU - Konovalova, Nina

PY - 1991

Y1 - 1991

N2 - Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four difTerent drugs (5-FU. mitomycin C, 4¢-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of3H-TdR and3H-UdR incorporation into DN A and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.

AB - Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four difTerent drugs (5-FU. mitomycin C, 4¢-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of3H-TdR and3H-UdR incorporation into DN A and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.

KW - 5-Fluorouracil

KW - Cell kinetics

KW - Chemosensitivity assays

KW - Colorectal cancer

KW - Mitomycin C

KW - Tumor chemotherapy

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Fractional incorporation of radionucleotides, a marker of in vitro tumor cell chemosensitivity in colorectal cancer

Abstract

Keywords

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