Fractional incorporation of radionucleotides, a marker of in vitro tumor cell chemosensitivity in colorectal cancer

Fabrizio Franchi, Patrizia Seminara, Diana Giannarelli, Nina Konovalova

Research output: Contribution to journalArticlepeer-review

Abstract

Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four difTerent drugs (5-FU. mitomycin C, 4¢-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of3H-TdR and3H-UdR incorporation into DN A and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.

Original languageEnglish
Pages (from-to)510-516
Number of pages7
JournalOncology
Volume48
Issue number6
DOIs
Publication statusPublished - 1991

Keywords

  • 5-Fluorouracil
  • Cell kinetics
  • Chemosensitivity assays
  • Colorectal cancer
  • Mitomycin C
  • Tumor chemotherapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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