Fractionated ionizing radiation exposure induces apoptosis through caspase-3 activation and reactive oxygen species generation

Barbara Bucci, Silvia Misiti, Annapaola Cannizzaro, Rodolfo Marchese, Giorgio H. Raza, Roberto Miceli, Antonio Stigliano, Donatella Amendola, Olimpia Monti, Michela Biancolella, Francesca Amati, Giuseppe Novelli, Aldo Vecchione, Ercole Brunetti, Ugo De Paula

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Background: Radiation therapy (RT) is a well established therapeutic modality for the treatment of solid tumors. In particular, post-operative RT is considered the standard treatment adjuvant to surgery since its ability to prolong median survival of patients with malignant astrocytoma has been shown; nevertheless the ionizing radiation (IR) treatment fails in a considerable number of astrocytoma patients. Materials and Methods: Using an ADF human astrocytoma cell line the molecular mechanisms involved in the DNA damage induced by fractionated irradiation (FIR) and single IR treatment have been investigated. Results: FIR and single IR treatment inhibited the growth of the ADF human astrocytoma cell line. FACS analysis revealed that FIR treatment, but not single IR treatment, induced growth inhibition associated with the induction of apoptosis. Apoptosis was related to caspase-3 activation and reactive oxygen species (ROS) generation. ROS formation depends on the up-regulation of the cytochrome P450 enzyme gene. On the contrary, 12.5 Gy induced necrotic cell death up-regulating the HSPD1, HSPCB, HSPCA and HSPB1 genes. Conclusion: FIR treatment induced cell death through caspase-3 and ROS-mediated apoptosis.

Original languageEnglish
Pages (from-to)4549-4557
Number of pages9
JournalAnticancer Research
Volume26
Issue number6 B
Publication statusPublished - Nov 2006

Fingerprint

Ionizing Radiation
Caspase 3
Reactive Oxygen Species
Apoptosis
Astrocytoma
Therapeutics
Cytochrome P-450 Enzyme System
Cell Death
Radiotherapy
Background Radiation
Radiation Exposure
Cell Line
Growth
Genes
DNA Damage
Up-Regulation
Survival

Keywords

  • Apoptosis
  • Astrocytoma
  • Microarray
  • Radiotherapy
  • ROS content

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bucci, B., Misiti, S., Cannizzaro, A., Marchese, R., Raza, G. H., Miceli, R., ... De Paula, U. (2006). Fractionated ionizing radiation exposure induces apoptosis through caspase-3 activation and reactive oxygen species generation. Anticancer Research, 26(6 B), 4549-4557.

Fractionated ionizing radiation exposure induces apoptosis through caspase-3 activation and reactive oxygen species generation. / Bucci, Barbara; Misiti, Silvia; Cannizzaro, Annapaola; Marchese, Rodolfo; Raza, Giorgio H.; Miceli, Roberto; Stigliano, Antonio; Amendola, Donatella; Monti, Olimpia; Biancolella, Michela; Amati, Francesca; Novelli, Giuseppe; Vecchione, Aldo; Brunetti, Ercole; De Paula, Ugo.

In: Anticancer Research, Vol. 26, No. 6 B, 11.2006, p. 4549-4557.

Research output: Contribution to journalArticle

Bucci, B, Misiti, S, Cannizzaro, A, Marchese, R, Raza, GH, Miceli, R, Stigliano, A, Amendola, D, Monti, O, Biancolella, M, Amati, F, Novelli, G, Vecchione, A, Brunetti, E & De Paula, U 2006, 'Fractionated ionizing radiation exposure induces apoptosis through caspase-3 activation and reactive oxygen species generation', Anticancer Research, vol. 26, no. 6 B, pp. 4549-4557.
Bucci B, Misiti S, Cannizzaro A, Marchese R, Raza GH, Miceli R et al. Fractionated ionizing radiation exposure induces apoptosis through caspase-3 activation and reactive oxygen species generation. Anticancer Research. 2006 Nov;26(6 B):4549-4557.
Bucci, Barbara ; Misiti, Silvia ; Cannizzaro, Annapaola ; Marchese, Rodolfo ; Raza, Giorgio H. ; Miceli, Roberto ; Stigliano, Antonio ; Amendola, Donatella ; Monti, Olimpia ; Biancolella, Michela ; Amati, Francesca ; Novelli, Giuseppe ; Vecchione, Aldo ; Brunetti, Ercole ; De Paula, Ugo. / Fractionated ionizing radiation exposure induces apoptosis through caspase-3 activation and reactive oxygen species generation. In: Anticancer Research. 2006 ; Vol. 26, No. 6 B. pp. 4549-4557.
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abstract = "Background: Radiation therapy (RT) is a well established therapeutic modality for the treatment of solid tumors. In particular, post-operative RT is considered the standard treatment adjuvant to surgery since its ability to prolong median survival of patients with malignant astrocytoma has been shown; nevertheless the ionizing radiation (IR) treatment fails in a considerable number of astrocytoma patients. Materials and Methods: Using an ADF human astrocytoma cell line the molecular mechanisms involved in the DNA damage induced by fractionated irradiation (FIR) and single IR treatment have been investigated. Results: FIR and single IR treatment inhibited the growth of the ADF human astrocytoma cell line. FACS analysis revealed that FIR treatment, but not single IR treatment, induced growth inhibition associated with the induction of apoptosis. Apoptosis was related to caspase-3 activation and reactive oxygen species (ROS) generation. ROS formation depends on the up-regulation of the cytochrome P450 enzyme gene. On the contrary, 12.5 Gy induced necrotic cell death up-regulating the HSPD1, HSPCB, HSPCA and HSPB1 genes. Conclusion: FIR treatment induced cell death through caspase-3 and ROS-mediated apoptosis.",
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AU - Bucci, Barbara

AU - Misiti, Silvia

AU - Cannizzaro, Annapaola

AU - Marchese, Rodolfo

AU - Raza, Giorgio H.

AU - Miceli, Roberto

AU - Stigliano, Antonio

AU - Amendola, Donatella

AU - Monti, Olimpia

AU - Biancolella, Michela

AU - Amati, Francesca

AU - Novelli, Giuseppe

AU - Vecchione, Aldo

AU - Brunetti, Ercole

AU - De Paula, Ugo

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