Fractionation and toxicity evaluation of waste waters

S. Galassi, E. Benfenati

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Several toxicity-based procedures have been proposed for waste water risk assessment but the toxic agents could never be identified in these very complex mixtures. A procedure was adopted using disposable solid-phase extraction cartridges to extract organic chemicals and preparative HPLC to fractionate them in relation of their hydrophobicity. Acute toxicity of whole samples and their fractions was measured on Daphnia magna, using a commercially available biokit. The procedure was applied to leachate from an industrial landfill and a textile effluent. In both cases the toxic effects due to xenobiotics were highest in the most hydrophobic HPLC fraction. The compounds responsible for the observed toxicity were identified and quantified by GC-MS. Reconstructed mixtures were analysed to assess their fitting with GC profiles and tested for toxicity to compare the responses of individual chemicals and mixtures. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalJournal of Chromatography A
Volume889
Issue number1-2
DOIs
Publication statusPublished - Aug 11 2000

Fingerprint

Fractionation
Waste Water
Toxicity
Wastewater
Poisons
Chemical Water Pollutants
High Pressure Liquid Chromatography
Organic Chemicals
Daphnia
Textiles
Solid Phase Extraction
Xenobiotics
Complex Mixtures
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Land fill
Risk assessment
Effluents

Keywords

  • Environmental analysis
  • Hydrophobicity
  • Phthalates
  • Toxicological analysis
  • Water analysis

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Fractionation and toxicity evaluation of waste waters. / Galassi, S.; Benfenati, E.

In: Journal of Chromatography A, Vol. 889, No. 1-2, 11.08.2000, p. 149-154.

Research output: Contribution to journalArticle

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