Free and protein-conjugated polyamines in mouse epidermal cells. Effect of high calcium and retinoic acid

M. Piacentini, N. Martinet, S. Beninati, J. E. Folk

Research output: Contribution to journalArticle

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Abstract

We have investigated polyamine metabolism in primary cultures of mouse epidermal cells. These cells, which grow at low Ca2+ levels as a monolayer with characteristics of basal cells, terminally differentiate when the extracellular Ca2+ level is raised above 1 mM. The cellular levels of free polyamines were measured, and, after incubation of cell cultures with [3H]putrescine, the distribution of label in both acid-soluble and acid-insoluble cellular components was examined. Free polyamine levels were reduced in cells induced to differentiate. Treatment with retinoic acid, which prevents differentiation and causes increased proliferation, resulted in an increase in free putrescine. Upon adjustment of the calcium concentration to a level that induces differentiation, the enzyme transglutaminase was activated, and a concomitant increase in the level of both protein-bound mono- and bis-γ-glutamyl derivatives of putrescine and spermidine was observed. Isolation of a material of apparent molecular weight about 6000 which contains only mono-γ-glutamylpolyamines and the finding of both mono- and bis-γ-glutamylpolyamines in the protein fraction containing cornified cell envelopes provided the basis for speculation on polyamines in envelope formation. Our data suggest that polyamines play a role during epidermal cell differentiation through transglutaminase-mediated post-translational modification.

Original languageEnglish
Pages (from-to)3790-3794
Number of pages5
JournalJournal of Biological Chemistry
Volume263
Issue number8
Publication statusPublished - 1988

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Polyamines
Tretinoin
Putrescine
Calcium
Transglutaminases
Proteins
Acids
Spermidine
Post Translational Protein Processing
Cell culture
Metabolism
Labels
Cell Differentiation
Monolayers
Cell Culture Techniques
Molecular Weight
Molecular weight
Cells
Derivatives
Enzymes

ASJC Scopus subject areas

  • Biochemistry

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Free and protein-conjugated polyamines in mouse epidermal cells. Effect of high calcium and retinoic acid. / Piacentini, M.; Martinet, N.; Beninati, S.; Folk, J. E.

In: Journal of Biological Chemistry, Vol. 263, No. 8, 1988, p. 3790-3794.

Research output: Contribution to journalArticle

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