We used a fluorescent dye, quin 2, to measure intracellular free calcium Ca+in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca++], level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca++](transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.
|Number of pages||5|
|Publication status||Published - 1988|
ASJC Scopus subject areas
- Arts and Humanities (miscellaneous)
- Clinical Neurology