Abstract
We used a fluorescent dye, quin 2, to measure intracellular free calcium Ca+in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca++], level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca++](transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.
Original language | English |
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Pages (from-to) | 476-480 |
Number of pages | 5 |
Journal | Neurology |
Volume | 38 |
Issue number | 3 |
Publication status | Published - 1988 |
ASJC Scopus subject areas
- Arts and Humanities (miscellaneous)
- Clinical Neurology
- Neuroscience(all)