Free cytoplasmic ca++ at rest and after cholinergic stimulus is increased in cultured muscle cells from duchenne muscular dystrophy patients

T. Mongini; D. Ghigo; C. Doriguzzi; F. Bussolino; G. Pescarmona; B. Polio; D. Schiffer; A. Bosia

Free cytoplasmic ca⁺⁺ at rest and after cholinergic stimulus is increased in cultured muscle cells from duchenne muscular dystrophy patients

T. Mongini, D. Ghigo, C. Doriguzzi, F. Bussolino, G. Pescarmona, B. Polio, D. Schiffer, A. Bosia

Research output: Contribution to journal › Article › peer-review

Abstract

We used a fluorescent dye, quin 2, to measure intracellular free calcium Ca+in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca⁺⁺], level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca⁺⁺](transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.

Original language	English
Pages (from-to)	476-480
Number of pages	5
Journal	Neurology
Volume	38
Issue number	3
Publication status	Published - 1988

ASJC Scopus subject areas

Arts and Humanities (miscellaneous)
Clinical Neurology
Neuroscience(all)

Cite this

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title = "Free cytoplasmic ca++ at rest and after cholinergic stimulus is increased in cultured muscle cells from duchenne muscular dystrophy patients",

abstract = "We used a fluorescent dye, quin 2, to measure intracellular free calcium Ca+in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca++], level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca++](transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.",

author = "T. Mongini and D. Ghigo and C. Doriguzzi and F. Bussolino and G. Pescarmona and B. Polio and D. Schiffer and A. Bosia",

year = "1988",

language = "English",

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T1 - Free cytoplasmic ca++ at rest and after cholinergic stimulus is increased in cultured muscle cells from duchenne muscular dystrophy patients

AU - Mongini, T.

AU - Ghigo, D.

AU - Doriguzzi, C.

AU - Bussolino, F.

AU - Pescarmona, G.

AU - Polio, B.

AU - Schiffer, D.

AU - Bosia, A.

PY - 1988

Y1 - 1988

N2 - We used a fluorescent dye, quin 2, to measure intracellular free calcium Ca+in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca++], level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca++](transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.

AB - We used a fluorescent dye, quin 2, to measure intracellular free calcium Ca+in cultured skeletal muscle cells and skin fibroblasts from five Duchenne muscular dystrophy (DMD) patients and from five controls. We observed an enhanced [Ca++], level, at rest and after acetylcholine (ACh) stimulation, in DMD muscle cells, but we did not detect any difference between DMD and normal skin fibroblasts. The abnormally higher [Ca++](transient induced by ACh suggests that it plays a critical role in muscle degeneration. The skin fibroblast results suggest that there is no generalized membrane defect.

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AN - SCOPUS:0023830725

VL - 38

SP - 476

EP - 480

JO - Neurology

JF - Neurology

SN - 0028-3878

IS - 3

ER -

Free cytoplasmic ca⁺⁺ at rest and after cholinergic stimulus is increased in cultured muscle cells from duchenne muscular dystrophy patients

Abstract

ASJC Scopus subject areas

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