Free radicals promote modifications in plasma high-density lipoprotein: Nuclear magnetic resonance analysis

Silvia Bradamante, Livia Barenghi, Gino A. Giudici, Carlo Vergani

Research output: Contribution to journalArticle

Abstract

The behavior of high-density lipoprotein (HDL) after free-radical-mediated oxidation was studied by incubating plasma HDL with chemical oxidizing systems (Cu++) in conditions similar to those used for low-density lipoprotein (LDL) chemical oxidation. Nuclear magnetic resonance (NMR) spectroscopy (1H and 31P) was used to evaluate the degree of oxidation and to characterize the oxidized products. The almost complete loss of polyunsaturated systems together with an appreciable decrease in choline peak demonstrates large-scale HDL-lipid degredation. The appearance of epoxide systems on fatty chains and the identification of oxidized cholesterol derivatives as cholesterol 5α,6β-epoxide, 7-keto, and 25-hydroxy confirm this picture. Phospholipid analysis indicates an alteration of the phospholipid profile in lyso-phosphatidylcholine (Lyso-PC) production and the disappearance of phosphatidylethanolamine (PE). This study shows that HDL is extensively degraded although there are no longer variations in the classical oxidative monitors, lipid hydroperoxide (LPO) and thiobarbituric acid reactive substance (TBARS). Our results suggest that HDL is significantly modified when submitted to an oxidative process.

Original languageEnglish
Pages (from-to)193-203
Number of pages11
JournalFree Radical Biology and Medicine
Volume12
Issue number3
DOIs
Publication statusPublished - 1992

Fingerprint

HDL Lipoproteins
Free Radicals
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Plasmas
Oxidation
Phospholipids
Thiobarbituric Acid Reactive Substances
Lipid Peroxides
Epoxy Compounds
Choline
Phosphatidylcholines
LDL Lipoproteins
Nuclear magnetic resonance spectroscopy
Cholesterol
Derivatives
Lipids

Keywords

  • Atherosclerosis
  • Free radicals
  • HDL
  • Lipid peroxidation
  • NMR
  • Nuclear magnetic resonance
  • Oxidatively modified lipoprotein

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine(all)
  • Toxicology

Cite this

Free radicals promote modifications in plasma high-density lipoprotein : Nuclear magnetic resonance analysis. / Bradamante, Silvia; Barenghi, Livia; Giudici, Gino A.; Vergani, Carlo.

In: Free Radical Biology and Medicine, Vol. 12, No. 3, 1992, p. 193-203.

Research output: Contribution to journalArticle

Bradamante, Silvia ; Barenghi, Livia ; Giudici, Gino A. ; Vergani, Carlo. / Free radicals promote modifications in plasma high-density lipoprotein : Nuclear magnetic resonance analysis. In: Free Radical Biology and Medicine. 1992 ; Vol. 12, No. 3. pp. 193-203.
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