Free solution mobility of DNA molecules containing variable numbers of cationic phosphoramidate internucleoside linkages

Nancy C. Stellwagen, Soffia Magnusdottir, John M. Dagle, Cecilia Gelfi, Pier Giorgio Righetti

Research output: Contribution to journalArticle

Abstract

The free solution electrophoretic mobility of an 118-base pair DNA fragment containing zero, three, six or nine cationic phosphoramidate internucleoside linkages has been measured by capillary electrophoresis. The electrophoretic mobility decreases with the increasing number of cationic phosphoramidate linkages, as expected because of the reduced negative charge on the DNA molecules. The decrease in mobility is approximately linear for DNA molecules containing three and six cationic phosphoramidate linkages, but begins to level off when nine cationic phosphoramidate linkages have been added. The mobility also varies somewhat depending on whether the modified phosphoramidate linkages are located at the 5'- or 3'-end of the DNA molecule. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalJournal of Chromatography A
Volume883
Issue number1-2
DOIs
Publication statusPublished - Jun 23 2000

Keywords

  • Capillary electrophoresis
  • DNA
  • Phosphoramidates

ASJC Scopus subject areas

  • Analytical Chemistry

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